Development and validation of an HPLC method for determination of carbamazepine in human plasma and applications to a therapeutic drug monitoring study

Authors : Emrah DURAL, Süleyman ÇETİN, Aslı BOLAYIR, Burhanettin ÇİĞDEM

Pages : 6-15

View : 46 | Download : 14

Publication Date : 2020-04-01

Article Type : Research Paper

Abstract :Background and Aims: Carbamazepine insert ignore into journalissuearticles values(CBZ); is an antiepileptic drug, which is prescribed as a first-line drug for the treatment of partial and generalized tonic–clonic epileptic seizures. The aim of this study was to develop and validate a simple, fast and reliable HPLC method for the determination of carbamazepine in human plasma. Methods: Chlorpromazine insert ignore into journalissuearticles values(CPR); was used as an internal standard. The separation was conducted with a C18 reverse-phase column insert ignore into journalissuearticles values(150x3.9 mm, 5 µm); at 30°C, using a mobile phase prepared with 20 mM KH2PO4, acetonitrile and methanol insert ignore into journalissuearticles values(6:3:1, v/v/v); by isocratic elution. Results: The method was linear between 0.5 and 40 µg/mL, determined by 10 individual calibration points. Total run time was ≤ 5 mins. Accuracy insert ignore into journalissuearticles values(RE%); values were determined between insert ignore into journalissuearticles values(-5.6); and 3.6%, and precision was determined at ≤4.2%. Limit of detection insert ignore into journalissuearticles values(LOD); was 0.04 µg/mL. The robustness test results of the method showed good values. Plasma CBZ of insert ignore into journalissuearticles values(n=30); those receiving CBZ quantities ranging from 0.2 to 1.2 g/day were measured with this method, and following analyses of their concentrations were found to be between 0.1 and 11.4 µg/mL insert ignore into journalissuearticles values(6.2±2.4 µg/mL);. While all plasma sample analyses were applied properly, it was observed that 16 insert ignore into journalissuearticles values(53.3%); of the plasma samples had CBZ lower than the recommended range. In addition to that, female patient plasma-CBZ levels were found significantly higher than male plasma contents insert ignore into journalissuearticles values(p<0.05);. Conclusion: This method was found suitable for the analysis of plasma samples collected during the therapeutic drug monitoring insert ignore into journalissuearticles values(TDM); of patients treated with CBZ.
Keywords : Carbamazepine, plasma, therapeutic drug monitoring, high performance liquid chromatography, ultraviolet detection

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