Nootropic herbal formulations for the treatment of Alzheimer’s disease: In vivo pharmacological assay and molecular docking studies

Authors : Naveen Kumar KOTLA, Shibnath KAMİLA, Shivani PATEL, Joel KOTHAPALLY, Aparna KONGARA, Satheesh MADHAV

Pages : 116-124

View : 42 | Download : 12

Publication Date : 2020-08-27

Article Type : Research Paper

Abstract :Background and Aims: The main aim of the study was to enhance the cognitive function of the brain by nootropic herbal formulations in animal models. Polyphyto herbal formulations were known to enhance the cognition and memory function by several pathways such as anti-oxidative, anti-inflammatory, and cell signaling pathways. In this study, six formulations were prepared by mixing specified plant parts and were coded as NHF1, NHF2, NHF3, NHF4, NHF5, and NHF6. Methods: The potency of the formulations was assessed by In vivo insert ignore into journalissuearticles values(photo actometer, rod walking test, pole climbing test, and Ellman’s acetylcholinesterase test); studies. Results: NHF1 and NHF5 exhibited greater activity than the standard drug donepezil in vivo insert ignore into journalissuearticles values(Ellman’s acetylcholinesterase test); analysis. NHF1 and NHF5 formulations containing plant parts were further investigated against several published literatures for the identification of chemical constituents and those chemical constituents were subjected to molecular docking and in silico ADME prediction studies to figure out the possible compounds responsible for the cholinesterase inhibition activity. Conclusion: In conclusion, the computational studies also reveal that presence of chemical constituents such as sarsasapogenin insert ignore into journalissuearticles values(13.13 nM);, racemosol insert ignore into journalissuearticles values(16.26 nM);, and beta-sitosterol insert ignore into journalissuearticles values(30.47 nM); having binding energy insert ignore into journalissuearticles values(-10.75 kcal/mol);, insert ignore into journalissuearticles values(-10.63 kcal/mol);, insert ignore into journalissuearticles values(-10.25 kcal/mol);, might be directly responsible for the nootropic activity.
Keywords : Herbal, nootropic, acetylcholinesterase, Alzheimer, autodock 4 2 6, sarsasapogenin, SwissADME

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