INVOLVEMENT OF MAIN OXIDATIVE STRESS MECHANISMS IN THE TOXICITY OF BENOMYL AND CARBENDAZIM IN RATS

Authors : S Catalgol, B Catalgol B ALPERTUNGA

Pages : 103-120

View : 33 | Download : 10

Publication Date : 2014-03-24

Article Type : Research Paper

Abstract :SUMMARY This study aimed to evaluate the role of oxidative stress in toxicity induced by BNL insert ignore into journalissuearticles values(benomyl); and its metabolite CBZ insert ignore into journalissuearticles values(carbendazim);, which are systemic broad-spectrum benzimidazole fungicides. The activities of superoxide dismutase insert ignore into journalissuearticles values(SOD);, catalase insert ignore into journalissuearticles values(CAT);, glutathione peroxidase insert ignore into journalissuearticles values(GSH-Px);, glutathione reductase insert ignore into journalissuearticles values(GSH-Rd);, γ−glutamyl transpeptidase insert ignore into journalissuearticles values(GGT); and glutathione-S-transferase insert ignore into journalissuearticles values(GST); as well as the levels of malonyldialdehyde insert ignore into journalissuearticles values(MDA);, being a lipid peroxidation marker and reduced glutathione insert ignore into journalissuearticles values(GSH); were measured in the liver, kidney, testis and brain tissues of the rats, after acute exposure of the two fungicides and their mixture. In all tissues, it was observed that MDA levels were increased and changes in antioxidant defense system were occurred. The induction of oxidative stress manifested as changes in the activity and/or levels of antioxidative parameters has been suggested as the mechanism by which BNL and CBZ induces their toxic effects. ÖZET Bu çalışmada, geniş spektrumlu sistemik benzimidazol grubu fungusidlerden olan BNL insert ignore into journalissuearticles values(benomyl); ve metaboliti CBZ insert ignore into journalissuearticles values(carbendazim);’ nin toksik etki mekanizmalarında oksidatif stresin rolü araştırıldı. BNL, CBZ ve karışımlarına akut maruziyet sonrası sıçanların karaciğer, böbrek, testis ve beyin dokularında oksidatif stres göstergesi olarak lipid peroksidasyon son ürünü malondialdehid insert ignore into journalissuearticles values(MDA); ve indirgenmiş glutatyon insert ignore into journalissuearticles values(GSH); seviyeleri, süperoksid dismutaz insert ignore into journalissuearticles values(SOD);, katalaz insert ignore into journalissuearticles values(CAT);, glutatyon peroksidaz insert ignore into journalissuearticles values(GSHPx);, glutatyon redüktaz insert ignore into journalissuearticles values(GSH-Rd);, gama glutamil transpeptidaz insert ignore into journalissuearticles values(GGT); ve glutatyon-S-transferaz insert ignore into journalissuearticles values(GST); antioksidan enzim aktivitelerinin tayini yapıldı. Tüm dokularda MDA düzeylerinin arttığı ve antioksidan savunma sistemlerinde değişiklik olduğu gözlendi. BNL ve CBZ’nin toksisitesinde, antioksidan parametrelerin aktivite ve/veya düzeylerinde oksidatif stresin indüksiyonu sonucu görülen değişikliklerin rol oynadığı sonucuna varıldı. Key words: Oxidative Stress, Benomyl, Carbendazim, Pesticide toxicity
Keywords : Oxidative Stress, Benomyl, Carbendazim, Pesticide toxicity

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