Nickel oxide nanoparticles induced DNA damages in human liver cells

Authors : Mahmoud ABUDAYYAK, E Elif GÜZEL, Gül ÖZHAN

Pages : 175-182

View : 37 | Download : 12

Publication Date : 2021-08-31

Article Type : Research Paper

Abstract :Background and Aims: Nickel oxide nanoparticles insert ignore into journalissuearticles values(NiO-NPs); are one of the most used nanoparticles, especially as photosensitizers. Although some studies evaluate their toxicity in the liver, the information about their toxicity at the cellular and molecular levels is still controversial. In the present study, it was aimed to investigate the in vitro toxic potentials of NiO-NPs insert ignore into journalissuearticles values(average size 15.0 nm); in the liver insert ignore into journalissuearticles values(HepG2); cell line. Methods: NiO-NPs were characterized by Transmission Electron Microscopy insert ignore into journalissuearticles values(TEM);, the cellular uptake of NPs and the morphologic changes were evaluated by TEM and Inductively Coupled Plasma-Mass Spectrometry insert ignore into journalissuearticles values(ICP-MS);, the cytotoxicity was evaluated by MTT and neutral red uptake insert ignore into journalissuearticles values(NRU); tests, comet assay was used for genotoxicity, Annexin V-FITC/propidium iodide insert ignore into journalissuearticles values(PI); apoptosis detection kit was used for apoptosis/ necrosis evaluation and Enzyme-Linked Immune Sorbent Assays insert ignore into journalissuearticles values(ELISA); kits were used for the potential of oxidative damage. Results: Our results showed that cellular uptake of NiO-NPs led to morphological changes in the cells, and caused cell death insert ignore into journalissuearticles values(IC50 was 146.7 μg/mL by MTT); mainly by apoptosis. Genotoxicity and oxidative damage were observed to be in a dosedependent manner. Conclusion: Results confirm previous data and draw attention to the toxic effects of NiO-NPs; further in vivo and in vitro studies need to be done to clarify the safety or toxicity of NiO-NPs.
Keywords : Nickel oxide, Nanoparticles, Genotoxicity, Oxidative stress, Apoptosis

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