Total phenolic, total flavonoid contents, and in vitro biological activities of Cephalaria procera Fisch. & Ave-Lall.

Authors : Nurdan YAZICI BEKTAŞ, Burak BARUT, Emel MATARACI KARA, Yeter YEŞİL

Pages : 365-371

Doi:10.26650/IstanbulJPharm.2021.1016208

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Publication Date : 2021-12-29

Article Type : Research Paper

Abstract :Background and Aims: This study aims to determine total phenolic, total flavonoid contents and in vitro biological activities of methanol insert ignore into journalissuearticles values(CEP-1);, n-butanol insert ignore into journalissuearticles values(CEP-2);, water insert ignore into journalissuearticles values(CEP-3);, n-hexane insert ignore into journalissuearticles values(CEP-4); extracts obtained from Cephalaria procera. Methods: The total phenolic and flavonoid content analysis, in vitro DPPH radical scavenging activities, cholinesterase, and tyrosinase inhibitory properties of the extracts were evaluated using spectrophotometric assays. DNA-damage and DNAdamage protective effects of the extracts were examined using agarose gel electrophoresis method. Antimicrobial activities of the extracts were determined by microdilution method. Results: CEP-3 had the best total phenolic content insert ignore into journalissuearticles values(79.64±1.11 mg GAE/g dry weight);, and CEP-1 had the highest total flavonoid content insert ignore into journalissuearticles values(15.33±0.27 mg QEE/g dry weight); among tested extracts. CEP-1 showed the highest radical scavenging activity with 83.21±3.20 μg/mL of IC50 value. CEP-3 exerted the highest AChE and BuChE inhibitory action with 134.63±4.49 μg/mL and 62.76±0.63 μg/mL of IC50 values, respectively. CEP-3 showed significant tyrosinase inhibitory action with 51.95±0.35 μg/mL IC50 value compared to kojic acid insert ignore into journalissuearticles values(58.26±0.25 μg/mL);. CEP-1 and CEP-3 were tested, and the both extracts did not damage supercoiled DNA at studied concentrations. Incidentally, results indicated that CEP-1 and CEP-3 protected supercoiled DNA against Fenton’s reagents. CEP-4 exhibited the highest antimicrobial activity on C. tropicalis with the MIC value of 156.2 μg/mL. Conclusion: The results showed that crude and subextracts of C. procera exerted several moderate activities on tested systems. It suggested that the species might be a promising medicinal plant for the treatment or prevention of several diseases associated with skin damage and wounds.
Keywords : Antioxidant, antimicrobial, anticholinesterase, Cephalaria procera, DNA protective, tyrosinase

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