Determination of the chemical composition, antioxidant potential of Sambucus ebulus L. (dwarf elder) fruit extracts and investigation of antimicrobial activity on Trichophyton rubrum (Castell.) Sabour and some microorganisms

Authors : Serpil DEMİRCİ KAYIRAN, Esra EROĞLU ÖZKAN, Emel MATARACI KARA, Nurdan YAZICI BEKTAŞ, Özden TARI, Merve NENNİ

Pages : 148-155

Doi:10.26650/IstanbulJPharm.2022.929762

View : 55 | Download : 10

Publication Date : 2022-08-30

Article Type : Research Paper

Abstract :Background and Aims: Sambucus ebulus L. is one of the medicinal plants well known in the traditional medicine of Anatolia since ancient times. The present study was aimed to investigate the antifungal potential of S. ebulus fruit extracts against Trichophyton rubrum insert ignore into journalissuearticles values(Castell.); Sabour as well as phytochemical composition, antibacterial, and antioxidant activities based on traditional usage. Methods: Two extracts were prepared from S. ebulus fruits. The phytochemical composition of S. ebulus fruit extracts was identified by LC-MS/MS. The antimicrobial activity was examined by using the broth microdilution method against a panel of microorganisms. In addition to this, S. ebulus extracts were evaluated for their in vitro antifungal activity against three yeast and T. rubrum by disc diffusion method. Results: The major compounds were determined in dried fruit methanol extract insert ignore into journalissuearticles values(DFM); as hederagenin insert ignore into journalissuearticles values(5.38±0.4949 μg/g); and fumaric acid insert ignore into journalissuearticles values(3.06±0.0275μg/g);. The fumaric acid insert ignore into journalissuearticles values(3.97±0.0357μg/g); was detected as the abundant compound in the fresh fruit juice insert ignore into journalissuearticles values(FFJ);. Acacetin, chrysin, eupatilin, hederagenin, isosakuranetin, myricitrin, and rhamnocitrin were detected in the extracts for the first time. DFM showed moderate activity against E. coli insert ignore into journalissuearticles values(MIC: 625 mg/L); and Candida tropicalis insert ignore into journalissuearticles values(MIC: 312.5 mg/L);. Both extracts possessed weak activity against Proteus mirabilis and Staphylococcus aureus,which had the MIC values 1250 mg/L. T. rubrum was found resistant to both extracts. The 2,2-diphenyl-1-picrylhydrazyl insert ignore into journalissuearticles values(DPPH); free radical cleaning method was used to measure the antioxidant capacity of the extracts. DFM and FFJ exhibited strong antioxidant activity against DPPH radicals with IC50 value of 5.941±0.236 μg/mL and 7.893±0.939 μg/mL, respectively. Conclusion: As a conclusion, although S. ebulus fruits are used to treat nail fungus insert ignore into journalissuearticles values(onychomycosis); by local folk, our results showed that it could not be useful to use in the antifungal topical formulations. In addition to this, the antibacterial activity result is parallel to the results of studies in this particular so far.
Keywords : Sambucus ebulus, LC MS MS, antifungal activity, Trichophyton rubrum, antioxidant activity

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