Anticholinesterase and antityrosinase activities of endemic Prangos heyniae H. Duman & M. F. Watson and its metabolites

Authors : Gökay ALBAYRAK, Serdar DEMİR, Halil KOYU, Sura BAYKAN

Pages : 51-57

Doi:10.26650/IstanbulJPharm.2023.1087126

View : 96 | Download : 122

Publication Date : 2023-04-28

Article Type : Research Paper

Abstract :Background and Aims: Prangos Lindl. insert ignore into journalissuearticles values(Apiaceae); are abundant in coumarins. Previously, along with n-hexaneinsert ignore into journalissuearticles values(HEX);, chloroforminsert ignore into journalissuearticles values(CHCl3);, and methanolinsert ignore into journalissuearticles values(MeOH); extracts, 8 molecules named ostholinsert ignore into journalissuearticles values(1);, isoimperatorininsert ignore into journalissuearticles values(2);, oxypeucedanininsert ignore into journalissuearticles values(3);, 7-methoxy-isoarnottinin-4\`-O-β-ᴅ-glucopyranosideinsert ignore into journalissuearticles values(4);, 7-methoxy-isoarnottinin-4\`-O-rutinosideinsert ignore into journalissuearticles values(5);, oxypeucedanin hydrate-3\`-O-β-ᴅ-glucopyranosideinsert ignore into journalissuearticles values(6);, 1-methylethyl-6-O-D-apio-β-ᴅ-furanosyl-β-ᴅ-glucopyranosideinsert ignore into journalissuearticles values(7);, and cnidioside Ainsert ignore into journalissuearticles values(8); were obtained from the roots of endemic Prangos heyniae H. Duman & M. F. Watson. 4 and 5 were reported as novel compounds. Coumarins are known for their neuroprotective properties. Tyrosinase and cholinesterase enzymes play a key role in the course of neurodegenerative diseases such as Parkinson’s and Alzheimer’s diseaseinsert ignore into journalissuearticles values(AD);, respectively. Therefore, we aimed to evaluate the antityrosinase and anticholinesterase effects of the extracts and compounds 1-8 from Prangos heyniae roots. Methods: Tyrosinase and acetylcholinesterase-butyrylcholinesteraseinsert ignore into journalissuearticles values(AChE-BChE); inhibitory activities of the samples were evaluated spectrophotometrically. The screening of the samples was carried on at 1000 μg/mL. Results of triplicate analyses of the samples were given as IC50 values obtained through linear regression analysis. Kojic acid and galantamine were used as positive controls for antityrosinase and anticholinesterase experiments, respectively. Results: Only MeOH extract showed antityrosinase activity with an IC50 value of 543.37±7.45 μg/mL. CHCl3 extract exhibited both AChE and BChE inhibitory activities with IC50 values of 273.92 ± 32.07 and 38.68±2.56 μg/mL, respectively. Among tested compounds, 6 showed a weak BChE-specific inhibitory activity insert ignore into journalissuearticles values(IC50= 91.93±3.86μg/mL); and managed to possess 40 times inferior activity than galantamineinsert ignore into journalissuearticles values(IC50= 2.25 ± 0.05μg/mL);. Conclusion: The CHCl3 extract displayed a good BChE inhibitory activity. These findings suggested that Prangos heyniae could be a valuable natural source to develop novel BChE inhibitors with further studies against AD.
Keywords : Prangos heyniae, coumarin, anticholinesterase activity, antityrosinase activity, oxypeucedanin hydrate 3

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