The effect of capsaicin on TBARS and TAS in rats with hypothyroidism

Authors : Fatih ERTEKİN, Tufan KEÇECİ

Pages : 98-104

Doi:10.30704/http-www-jivs-net.1137848

View : 19 | Download : 9

Publication Date : 2022-08-31

Article Type : Research Paper

Abstract :In this study, capsaicin was administered to rats with experimental hypothyroidism. İt was aimed to determine the changes in plasma levels of thiobarbituric acid reactive substances insert ignore into journalissuearticles values(TBARS);, which are indicators of oxidative stress, and total antioxidant capacity insert ignore into journalissuearticles values(TAS);, which is one of the components of antioxidant defence mechanisms. A total of 32 healthy male Wistar Albino rats weighing 300-350 g, approximately 12 weeks old, were used as animal material in the study. Rats were divided into four equal groups control insert ignore into journalissuearticles values(K);, Capsaicin insert ignore into journalissuearticles values(C);, Hypothyroid insert ignore into journalissuearticles values(H); and capsaicin + Hypothyroid insert ignore into journalissuearticles values(CH);. During the 30-day trial period, insert ignore into journalissuearticles values(10mg / kg / day); capsaicin was administered to the rats in group C by oral gavage per animal. In group H, 6-n-propyl-2-thiouracil insert ignore into journalissuearticles values(PTU); was added daily to their drinking water at 0.05% weight/volume insert ignore into journalissuearticles values(W/V);. In the CH group, 10 mg/kg/day of capsaicin was administered by oral gavage method and 0.05% weight / volume insert ignore into journalissuearticles values(W / V); of PTU was added to drinking water. At the end of the application, we obtained plasma and serum samples from the subjects in the groups under general anaesthesia insert ignore into journalissuearticles values(thiopental anaesthesia, 40 mg/kg); and by taking sufficient amount of blood from the heart by cardiac puncture. We determined thyroid-stimulating hormone insert ignore into journalissuearticles values(TSH);, total triiodothyronine insert ignore into journalissuearticles values(TT3);, free T3 insert ignore into journalissuearticles values(fT3);, total thyroxine insert ignore into journalissuearticles values(TT4); and free T4 insert ignore into journalissuearticles values(fT4); levels from serum samples, and TBARS and TAS levels from plasma samples. In conclusion, in the light of the data obtained in this study, we determined that lipid peroxidation and oxidative stress occur in hypothyroidism. However, we concluded that the application of capsaicin is partially sufficient to maintain the oxidant/antioxidant balance.
Keywords : Antioxidant, Capsaicin, Hypothyroidism, Oxidative stress, Rat