The basic principles of cryopreservation and the importance of polyampholytes as a cryoprotectant

Authors : Haydar Uğur, Saadet Kevser Pabuccuoğlu, Serhat Pabuccuoğlu

Pages : 47-56

View : 27 | Download : 31

Publication Date : 2025-04-30

Article Type : Review Paper

Abstract :Cryopreservation is an important process used to store the materials such as biological samples and food in liquid nitrogen (-196 °C) or in ultralow temperature freezer (-86 °C) for various biomedical, clinical and food-related applications. The main goal in the cryopreservation is to protect the materials against to damages during the freeze-thaw steps. Polyampholytes, which are the polymers containing both the cationic and anionic groups, have emerged as promising cryoprotective agents due to their unique properties. This review comprehensively discusses the history of cryopreservation, its fundamental principles, and the synthesis and cryoprotective properties of polyampholytes, with a focus on their mechanisms and applications. .
Keywords : Polyampholytes, cell cryopreservation, cryoprotectant, non-permeable cryoprotectant