Toothpaste with Propolis “Apident” Shows Antimicrobial Activity in vitro

Authors : Klemen RIHAR, Dunja Gregorič EXEL, Adriana PEREYRA, Rok KOPINČ, Bratko FILIPIČ

Pages : 31-31

View : 13 | Download : 11

Publication Date : 2018-12-08

Article Type : Conference Paper

Abstract :In this study, we evaluated the antibacterial activity of toothpaste with Propolis, used for the treatment of teeth and as a medicine for endodontic treatment. Toothpaste with Propolis was prepared using the water-soluble Propolis extract. Calcium Hydroxide was a control, as well as two commercial and two medical toothpastes. The bacteria on which Propolis toothpaste works are Streptococcus mutans, Staphylococcus aureus, Micrococcus luteus, Escherichia coli, Pseudomonas aeruginosa and yeast Candida albicans . The inhibition of bacterial/yeast growth was evaluated by colouring with resazurine. The inhibition of bacterial/yeast growth is apparent in changing the color of resazurine from blue to pink. MIC insert ignore into journalissuearticles values(Minimum Inhibitory Concentration); was determined as the maximum dilution in which no minimal color change occurs or a minimal color change occurs. For example, in the seventh hole, the color from pink to blue has changed. Therefore, the color change in the seventh hole at dilution 1:64 means MIC = 1 × 10/64 = 0.156 mg/mL. MIC values ​​ of toothpaste with Propolis were against S. aureus : 0.031 mg/mL insert ignore into journalissuearticles values(0.326 mg/mL of medical toothpaste, 0.187 mg/mL of commercial toothpaste);; Micrococcus luteus : 0.003 mg/mL insert ignore into journalissuearticles values(0.140 mg/mL of medicinal toothpaste, 0.046 mg/mL of commercial toothpaste);; Streptococcus mutans : 0.015 mg/mL insert ignore into journalissuearticles values(0.140 mg/mL medical toothpaste, 0.019 mg/mL of commercial toothpaste);; E. coli : 0.015 mg/mL insert ignore into journalissuearticles values(0.375 mg/mL medical toothpaste, 0.062 mg/mL of commercial toothpaste);; Pseudomonas ae ruginosa : 0.062 mg/mL insert ignore into journalissuearticles values(0.281 mg/mL medical toothpaste, 0.046 mg/mL of commercial toothpaste); and yeast Candida albicans : 0.015 mg/mL insert ignore into journalissuearticles values(0.140 mg/mL medical toothpaste, 0.281 mg/mL commercial toothpaste );. Comparison of MIC values ​​ between toothpaste with Propolis and medical toothpastes demonstrates greater effectiveness of Propolis toothpaste from 4.53 to 25.0 times. For St. aureus , this was 10.51 times; for Str. mutans 9.33 times, E. coli 25.0 times; for Pseudomonas aeruginosa 4.53 times and yeast Candida albicans 9.33 times. Compared with commercial toothpastes, Propolis toothpaste was better: 0.74 to 18.73 times. For St. aureus , was 6.03 times, for Str. mutans 1.26 times, for E. coli 4.13 times; for Pseudomonas aeruginosa 0.74 times and yeast Candida albicans 18.73 times.  
Keywords : Propolis, Tooth paste, Antimicrobial Activity, in vitro