Chemical Profiling of Papua New Guinea Propolis and Assay its Antiprotozoal Activity

Authors : Samya ALENEZI, Naif Alenezi MANAL, J NATTO, Harry P De KONING, John IGOLI, David G WATSON

Pages : 35-35

View : 13 | Download : 9

Publication Date : 2018-12-08

Article Type : Conference Paper

Abstract :This study is aimed to investigate the chemical profile and examine the in vitro efficacy of Papua new guinea insert ignore into journalissuearticles values(PNG); propolis and compounds purified from it purified compounds against the protozoal parasites: Crithidia fasciculate, Trypansoma congolense, Trypanosoma b. brucei and drug resistant Trypanosoma brucei insert ignore into journalissuearticles values(B48);, which cause diseases in humans and other animals as well as insects. In addition. The samples were assayed for their toxicity against human U937 cells in vitro .              Chemical profiling was conducted by using negative ion spray ESI insert ignore into journalissuearticles values(LC-MS); and indicated that the ethanolic extract of PNG contained a series of triterpenes. Principle components analysis insert ignore into journalissuearticles values(PCA); of the processed LC-MS data collected was demonstrated the uniqueness in chemical composition of the fractions prepared from the sample that were also active against the parasites . Active principles were isolated by bioassay-led fractionation, using medium pressure chromatographic insert ignore into journalissuearticles values(MPLC);. Afterwards, by using spectroscopic and spectrometric means, nine pure compounds were isolated and the their structures were elucidated indicating that eight of them were cycloartane type triterpenes. While the other one was a pentacyclic triterpene, 20 hydroxy betuline that was found to be the most active compound in these assays. Growth curves of drug-resistant T. brucei at concentrations ≥ EC50 showed that this compound was trypanostatic. Overall, the PNG propolis and its fractions showed low toxicity on U937 cell growth and were efficacious against kinetoplastid parasites. 
Keywords : Papua New Guinea Propolis, Chemical Profiling, Antiprotozoal Activity