Preliminary Studies: The Potential Anti-Angiogenic Activities of Two Sulavesi Island (Indonesia) Propolis and Their Chemical Characterization

Authors : Muhammad IQBAL, Taiping FAN, David WATSON, Sameah ALENEZI, Muhamad SAHLAN

Pages : 49-49

View : 12 | Download : 8

Publication Date : 2018-12-08

Article Type : Conference Paper

Abstract :Several studies have previously reported propolis, or its constituents, to inhibit tumour angiogenesis 1 .   The anti-angiogenic activity of two Indonesian stingless bee propolis extracts from Sulawesi Island on vascular cells were assessed. Sample D01 was obtained from the outer side of bee hives, while D02 was from the inner side of the same hives. Preliminary results of cell viability and multi-channel wound healing assays o n HUVECs and placenta-derived pericytes demonstrated that D01 elicited a strong cytotoxic effect and a considerable anti-migratory activity on the vascular cells. Although D02 demonstrated a much weaker cytotoxic effect on the cell lines compared to D01, it   elicited a substantial   protective   effect on the pericytes   against CoCl 2 -induced dropout in an experiment to mimic a micro - environment commonly associated with angiogenesis and tumour growth 2 . These   results demonstrate modulatory effects of   these   propolis   samples   in vascular cells, which requires further investigation . The extracts were profiled by using liquid chromatography coupled to high resolution mass spectrometry . The most abundant compounds in Indonesian sample D 0 1 from LC-MS analysis seem to be unusual since they do not immediately fall into a clear class such as flavonoids or terpenoids. Two of the most abundant compound s have elemental compositions matching actinopyrones which are antibiotic compounds isolated from Streptomyces pactum 3 . Identification on the basis of elemental composition is not definitive but compounds in D 0 1 are possibly due to unusually modified terpenoids. Sample D 0 2 has abundant compounds which include four related diterpenes with differing degrees of oxygenation and some sesquiterpenes. However, again the profile is unusual. 
Keywords : Anti Angiogenic Activities, Propolis