Cytotoxic Activity of Four Propolis Colombian Samples Against Canine Osteosarcoma Cells

Authors : Dolly Patricia Pardo MORA, Oscar Julián MURILLO, Mauricio Rey BUITRAGO, Mónica LOSADA, Jaime Fabian Cruz URIBE, Karina Basso SANTIAGO, Bruno José CONTI, José Maurício SFORCIN

Pages : 57-57

View : 13 | Download : 13

Publication Date : 2018-12-08

Article Type : Conference Paper

Abstract :The cytotoxicity of propolis against tumor cells has been widely reported 1 . Propolis from tropical areas present components such as triterpenes, diterpenes and benzophenones 2 , however, new types of propolis may be recognized and their biological activities need to be elucidated. Therefore, four Colombian propolis samples were chemically characterized by gas chromatography-mass spectrometry insert ignore into journalissuearticles values(GC–MS); and their cytotoxic action against canine osteosarcoma insert ignore into journalissuearticles values(OSA); cells was determined, as well as apoptosis induction and changes in gene expression of CASPASE 8 and CASPASE 9. OSA cells were treated with ethanolic extracts of propolis; the lactate dehydrogenase insert ignore into journalissuearticles values(LDH); assay and the annexin V/propidium iodide kit were used to evaluate the cytotoxic effect and apoptosis induction, respectively. Changes in gene expression were evaluated by quantitative real time PCR insert ignore into journalissuearticles values(qPCR);. The propolis samples were rich in diterpenes, triterpenes and benzophenones. Propolis samples induced a cytotoxic effect towards OSA cells that was correlated to the LDH leakage into the culture medium after cell membrane rupture. The cytotoxic effect increased in a time-concentration manner after incubation with propolis and the cytotoxic concentration 50% insert ignore into journalissuearticles values(CC 50 ); was lower than 36.24 µg/ml after 72 h of treatment. Colombian propolis samples induced total apoptosis significantly insert ignore into journalissuearticles values(untreated cells: 10.77 ± 2.22%; treated cells: from 58.403% ±1.39 to 95.97 ± 2.14% after 48 h);. All propolis samples increased the expression of CASPASE 8 and three of them induced the expression of CASPASE 9. Colombian propolis samples induced apoptosis in canine OSA cells, suggesting their promising potential as news strategies for cancer therapy. Acknowledgements: Universidad Antonio Nariño, Bogotá Colombia
Keywords : Cytotoxic Activity, Propolis, canine osteosarcoma OSA, cells