Antioxidant Activities of Propolis from Aragón (Spain)

Authors : M Teresa SANCHO, Sandra M OSÉS, Andrea RODRÍGUEZFERNÁNDEZ, Miguel A FERNÁNDEZMUIÑO, Consuelo PÉREZARQUILLUÉ, Regina LÁZARO, Susana BAYARRI

Pages : 67-67

View : 14 | Download : 12

Publication Date : 2018-12-08

Article Type : Conference Paper

Abstract :Propolis has demonstrated a strong antioxidant activity 1 . This property, together with other compositional parameters 2 could contribute to the difficult standardization of propolis. Antioxidant activity should be determined by methods based on different mechanisms, and against several free radicals 3 . The purpose of this research was to help characterize propolis from Aragón insert ignore into journalissuearticles values(Spain); by determining the oxygen radical absorbance capacity insert ignore into journalissuearticles values(ORAC); 4 , trolox equivalent antioxidant capacity insert ignore into journalissuearticles values(TEAC); 5 , 2,2-diphenyl-1-picrylhydrazyl insert ignore into journalissuearticles values(DPPH); assay 6 , as well as the radical-scavenging effect on hydroxyl radicals insert ignore into journalissuearticles values(AOA); 5 on hydroalcoholic extracts of eight Aragonese propolis samples. Propolis extracts were obtained with 70% insert ignore into journalissuearticles values(V/V); ethanol. All extracts showed high antioxidant activities. The following averages were obtained: For ORAC 11070 µmol trolox/g, for TEAC 1040.7 µmol trolox/g, for IC 50 DPPH 0.0698 mg/ml and for AOA 0.8783 mmol uric acid/g. At the 95.0% confidence level, significant linear correlations were found between ORAC and TEAC insert ignore into journalissuearticles values(r= 0.9436);, ORAC and AOA insert ignore into journalissuearticles values(r= 0.9142); and TEAC and AOA insert ignore into journalissuearticles values(r= 0.9919); and a double reciprocal correlation between TEAC and DPPH insert ignore into journalissuearticles values(r= 0.7403);. The lack of correlation between ORAC and AOA with DPPH results is likely due to the fact that the antioxidant compounds of Aragonese propolis could react very fast with both peroxyl insert ignore into journalissuearticles values(ORAC assay); and hydroxyl insert ignore into journalissuearticles values(AOA assay); radicals, and at a slow pace to DPPH due to steric inaccessibility 3 . Nevertheless, further studies are necessary to corroborate this conclusion.   Acknowledgements: To all the beekeepers who have provided with a free sample of propolis for this study.
Keywords : Propolis