Antituberculosis Activity of Propolis

Authors : Jarosław WIDELSKI, Joanna GOLUS, Piotr OKIŃCZYC, Rafał SAWICKI, Grażyna GINALSKA, Tomasz MROCZEK, Zuriyadda SAKIPOVA, İoanna CHINOU, Krystyna SKALICKAWOŹNIAK

Pages : 77-77

View : 10 | Download : 14

Publication Date : 2018-12-08

Article Type : Conference Paper

Abstract :Tuberculosis an infectious disease caused by Mycobacterium tuberculosis is one of the leading causes of human morbidity and mortality. In 2013 the WHO reported one and half million of deaths and nine millions new cases of active tuberculosis caused by TB 1 .The increase in the incidence of clinical tuberculosis is associated with increasing reports of new cases of multi drug resistant insert ignore into journalissuearticles values(MDR-TB); and extensively multidrug resistant insert ignore into journalissuearticles values(XDR-TB); strains 1 . Propolis insert ignore into journalissuearticles values(bee glue); is well known for its antibacterial and antifungal properties.More than 30 ethanol-water extracts obtained from different propolis samples were tested for antituberculosis activity. Minimal Inhibitory Concentrations insert ignore into journalissuearticles values(MIC); values for the EO were established with 96-well micro plate method with AlamarBlue insert ignore into journalissuearticles values(Invitrogen);. Reference strain of Mycobacterium tuberculosis H37Ra inoculum in Middlebrook 7H9 broth insert ignore into journalissuearticles values(Difco); was 5x10 5 cfu/ml per well, accordingly to CLSI standards. Serial twofold dilutions of propolis extracts ranged from 64 to 8 µg/ml. As the internal control of the method serial twofold dilutions of four first line antibiotics dedicated to the tuberculosis treatment: isoniazid insert ignore into journalissuearticles values(INH);, rifampicin insert ignore into journalissuearticles values(RMP);, ethambutol insert ignore into journalissuearticles values(EMB); and streptomicin insert ignore into journalissuearticles values(SM); were used 2,3 . Acknowledgments. The work was financed from grant No 4/POLTUR-1/2016.
Keywords : Propolis, Mycobacterium tuberculosis, MIC