Usability of the phenolic profile analysis method developed in RP-HPLC-PDA in natural products

Authors : Yakup Kara, Ceren Birinci

Pages : 14-27

Doi:10.35206/jan.1430767

View : 64 | Download : 64

Publication Date : 2024-06-30

Article Type : Research Paper

Abstract :The predominant majority of bioactive compounds in natural products are polyphenols. Reverse Phase High Performance Liquid Chromatography (HPLC) is the most employed analytical method for determining the polyphenol profiles of natural products. Analyses are conducted based on methods validated according to the number and type of phenolic standards used. In this study, it was aimed to determine 26 phenolic compound standards with HPLC-fotodiot array (PDA) detector, which is preferred for the separation of secondary metabolites commonly found in natural products. The analysis was carried out utilizing a C18 column (250 mm x 4.6 mm, 5 μm; GL Sciences) with a gradient program. The HPLC method was developed, determining the limit of detection within the range of 0.019-0.072 μg/mL, and the limit of quantification within the range of 0.063-0.239 μg/mL. All calibration curves exhibited linear corelations with R² values exceeding 0.994 across the specified range. The developed method has been optimized and validated by assessing detection and quantification limits, accuracy, repeatability, and recovery data suitable for phenolic analysis. It has been concluded that the optimized method allows for the rapid and reliable evaluation of the phenolic content of natural products and their quantitative determination.
Keywords : Phenolic content, Bee products, Plant, HPLC