Melatonin regulation of some important metabolic enzymes during intestinal ischemia/reperfusion injury in rats

Authors : H Ramazan Yılmaz, Burhan Ates, Eşref Yüksel, İsmet Yılmaz, Mustafa Iraz

Pages : 70-77

View : 17 | Download : 23

Publication Date : 2013-08-13

Article Type : Research Paper

Abstract :Intestinal ischemia/reperfusion insert ignore into journalissuearticles values(I/R); injury is known to result from both tissue hypoxia and the consequences of reperfusion of ischemic tissues. In addition, ATP stores are depleted during ischemia because of the relatively inefficient production of ATP by glycolysis. In this study, the effect of melatonin on some important metabolic enzymes hexokinase insert ignore into journalissuearticles values(HK);, glucose-6-phosphate dehydrogenase insert ignore into journalissuearticles values(G6PD);, lactate dehydrogenase insert ignore into journalissuearticles values(LDH); and malate dehydrogenase insert ignore into journalissuearticles values(MDH); responsible for generation of energy was investigated. Melatonin was determined to cause a distinct regulation of these enzymes. While the activity of the enzyme HK of the first step of glycolysis was significantly higher in pre-melatonin treated I/R group than group I/R animals, the activities of LDH and G6PD were decreased markedly with melatonin application. Although, the MDH levels of pre-melatonin treated I/R group were higher than the sham control group, this might not be a result of melatonin application, since both I/R and I/insert ignore into journalissuearticles values(Mel/R); groups also showed even higher MDH activity than the control group.
Keywords : ischemia reperfusion, melatonin, hexokinase, glucose 6 phosphate dehydrogenase, malate dehydrogenase, lactate dehydrogenase