The effects of CAPE on oxidative stress and histopathological values in rats treated with subacute dichlorvos

Authors : Gökhan NUR, Aysel GÜVEN, Pınar AKSU KILIÇLE

Pages : 647-655

Doi:10.37212/jcnos.418660

View : 16 | Download : 8

Publication Date : 2018-04-26

Article Type : Research Paper

Abstract :This study was designed to investigate the  protective effect of CAPE against dichlorvos induced  hepatotoxicity in rats. 40 rats were used in the study,  including 10 in each group. Corn oil of 5 mg/kg  insert ignore into journalissuearticles values(solvent); was administered intragastrically to rats in the  group 1, dichlorvos of 5 mg/kg was dissolved in corn oil  and administered to rats in the group 2 via gavage,  dichlorvos insert ignore into journalissuearticles values(5 mg/kg, via gavage route); and CAPE insert ignore into journalissuearticles values(10  μM/kg, intraperitoneally); were administered to rats of  the group 3 with one-hour interval providing that CAPE  treatment was started three days earlier than dichlorvos  administration. The rats in the group 4 were administered with 10%  solvent ethanol via intraperitoneally. All administrations  continued for 15 days and blood of animals was drawn  via intracardiac route after they were sacrificed by  cervical dislocation under ketamine/xylazine anesthesia.  Total antioxidant, total oxidant, and paraoxonase levels  were measured from the obtained plasma samples. As a  result of dichlorvos administration, paraoxonase activity  and total antioxidant levels decreased; whereas, total  oxidant levels increased. Histopathological analysis  revealed that liver tissue appeared normal in control  groups insert ignore into journalissuearticles values(1 and 4);; on the other hand, there were  degeneration, congestion, cellular infiltration, and  necrotic areas in the group administered with  dichlorvos. Even though frequency of lesions decreased,  similar lesions were observed in the group with  Dichlorvos+CAPE. Consequently, it was determined  that while dichlorvos administration increased oxidative  stress, CAPE administration had a protective potential  increasing antioxidant capacity.
Keywords : Dichlorvos, CAPE, Hepatotoxicity, Oxidative stress, Subacute administration