The Effects of Delayed Fluid Resuscitation on Lung Oxidative Stress and Antioxidant Vitamin Levels in Controlled Hemorrhagic Shock: An Experimental Study

Authors : Hacı Ahmet BİRCAN

Pages : 429-438

Doi:10.37212/jcnos.607125

View : 25 | Download : 14

Publication Date : 2015-09-01

Article Type : Research Paper

Abstract :We aimed to determine the effects of delayed fluid resuscitation on the lung oxidative stress and antioxidant vitamin levels in a rat model of controlled hemorrhagic shock insert ignore into journalissuearticles values(HS);. Male Wistar rats were exposed to controlled HS via arterial catheterization to reduce mean arterial pressure insert ignore into journalissuearticles values(MAP); to 40 mmHg over 10 minutes. Two groups were constituted according to resuscitation time: early insert ignore into journalissuearticles values(n=6); and delayed insert ignore into journalissuearticles values(n=5);, respectively resuscitated 30 or 90 minutes after HS. A control group insert ignore into journalissuearticles values(n=5); was subjected to catheterization only. I ntravenous f luid resuscitation was done with R inger lactate solution. After 24 hours, b ronchoalveolar lavage insert ignore into journalissuearticles values( BAL ); was performed and the lungs were harvested for biochemical, cytological and histopathological analyses. Lipid peroxidation insert ignore into journalissuearticles values(as MDA);, reduced glutathione insert ignore into journalissuearticles values(GSH);, glutathione peroxidase insert ignore into journalissuearticles values(GSH-Px); and vitamin A, vitamin C and vitamin E levels were measured in both BAL fluid insert ignore into journalissuearticles values(BALF); and lung homogenate. Lung tissue GSH-Px and vitamin E levels are increased in both HS groups compared to the control group. No significant differences were found in MDA, GSH, vitamin A and vitamin C BALF levels among all groups, except for GSH-Px insert ignore into journalissuearticles values(p=0.007);. Intracellular antioxidants, especially GSH-Px and vitamin E, increase in the lungs of rats insert ignore into journalissuearticles values(in both HS groups);, possibly due to increased oxidative stress and increased physiological requirements after HS and resuscitation.
Keywords : Antioxidants, Hemorrhagic shock, Lung, Oxidative stress, Vitamin