The high co-existence rate of Blastocystis and Dientamoeba fragilis in human faecal samples and the analysis of demographic and clinical findings

Authors : Erdogan MALATYALİ, İbrahim YILDIZ, Evren TİLEKLİOGLU, Hatice ERTABAKLAR, Sema ERTUĞ

Pages : 422-427

Doi:10.32322/jhsm.904858

View : 31 | Download : 10

Publication Date : 2021-07-15

Article Type : Research Paper

Abstract :Aim: Blastocystis and Dientamoeba fragilis insert ignore into journalissuearticles values(D. fragilis); are among the most common protozoon species in human faecal samples. The cross-sectional studies have reported the frequencies in a variety of populations. However, we have very limited information about the co-existence rate of those protozoans. The study aimed to compare D. fragilis frequency in Blastocystis positive and negative faecal samples in order to determine the co-existence rate. The secondary objective was to analyse demographic characteristics and gastrointestinal insert ignore into journalissuearticles values(GI); symptoms in relation to both infections. Material and Method: In the present study, we defined a study group that included 100 Blastocystis positive faecal samples and a control group that included 100 Blastocystis negative samples. The frequency of D. fragilis in samples was determined with a PCR assay specific to the small-subunit ribosomal RNA insert ignore into journalissuearticles values(SS rRNA); gene. A positive control of D. fragilis was used and the samples with amplification of the expected size insert ignore into journalissuearticles values(863 bp); were considered as positive. In addition to the statistical comparison of frequencies, the descriptive and clinical findings of cases were analysed retrospectively with Pearson chi-square or ANOVA tests. Results: The frequency of D. fragilis was 21% in Blastocystis positive group and it was 10% in Blastocystis negative group. There was statistically significant difference in terms of D. fragilis positivity between the groups insert ignore into journalissuearticles values(p 0.05);. Conclusion: Our study highlighted the high co-existence of D. fragilis and Blastocystis in human faecal samples. A possible explanation for this finding may be the faecal-oral transmission of these protozoans. In addition, analysis of clinical findings was supported common asymptomatic colonisation of Blastocystis and D. fragilis.
Keywords : Blastocystis, Dientamoeba fragilis, co infection, clinical findings