Investigation of the effect of watercress via nuclear factor-E2-related factor-2 pathway on 7,12-dimethylbenz[a]anthracene-induced oxidative damage in rat liver

Authors : Kübra ATAY, Ayşe Burçin UYUMLU, Basri SATILMIŞ, Aslı ÇETİN, Hatice ÇAĞLAR YILMAZ

Pages : 936-942

Doi:10.32322/jhsm.946969

View : 29 | Download : 13

Publication Date : 2021-09-24

Article Type : Research Paper

Abstract :Aim: Nuclear factor E2-related factor-2 insert ignore into journalissuearticles values(Nrf2); regulates many cytoprotective antioxidants, and detoxifying enzymes, has been a hopeful approach for chemoprevention of cancer. We researched the chemopreventive impact of watercress on the DMBA-induced oxidative damage in the rat liver via the Nrf2/ARE signalling pathway in the present study. Material and Method: Female Wistar albino rats have categorized into three groups. The control was the first group, rats in the second and third groups were administered 7,12-dimethylbenz[a]anthracene insert ignore into journalissuearticles values(DMBA); insert ignore into journalissuearticles values(20 mg/kg b.w., i.p.); and rats in the third group were given daily doses of watercress insert ignore into journalissuearticles values(250 mg/kg b.w. Nasturtium officinale R.Br. for 4 weeks by oral gavage);. Antioxidant and phase II detoxification enzyme activities, Nrf2 transcription factor level in liver, and serum ALT were determined. Also, histopathological analysis of the liver was performed. Results: We observed that watercress induces DNA-binding of Nrf2. It was related to increased enzyme activities of phase II detoxifying and the antioxidant. Our results also demonstrated that watercress ameliorated liver injury. Conclusion: Our data ensured considerable evidence that the dietary watercress ameliorates DMBA induced liver toxicity via regulating the activation of the Nrf2/ARE pathway which increases the expression of cytoprotective enzymes.
Keywords : Watercress, Nrf2, DMBA, Xenobiotic enzymes, Hepatoprotective effect