Identification of lymphocyte subgroups with flow cytometry in COVID-19 patients

Authors : İlhami BERBER, Nurcan KIRICI BERBER, Ahmet SARICI, Harika GÖZÜKARA BAĞ, Soykan BİÇİM, Burhan TURGUT, Furkan ÇAĞAN, Mehmet Ali ERKURT, Ayşe UYSAL, Nihal Sümeyye ULUTAŞ, Emin KAYA, İrfan KUKU

Pages : 1183-1189

Doi:10.32322/jhsm.1129894

View : 23 | Download : 9

Publication Date : 2022-07-20

Article Type : Research Paper

Abstract :Objective: We aimed to determine lymphocyte subgroups and activation status of flow cytometry in COVID-19 patients and examine their relationship with disease stage and length of hospital stay. Material and Method: Forty patients were analyzed in this study and compared with the age and sex-matched 40 healthy controls. COVID-19 patients have split as early and advanced-stage diseases. Flow cytometry assay was performed to determine the counts of lymphocyte subsets and activation status. Total lymphocyte count was calculated and CD45 insert ignore into journalissuearticles values(cluster of differentiation);, CD3, CD4, CD8, CD19, CD27, CD38, CD56, CD57, and IgD were studied on lymphocyte gate. T helper / T cytotoxic rates and length of hospital stay were recorded. Results: The patients` CD3insert ignore into journalissuearticles values(+);CD4insert ignore into journalissuearticles values(+); insert ignore into journalissuearticles values( T helper); count and CD27 expression on T cells counts were significantly lower, and CD57 expression on CD3insert ignore into journalissuearticles values(+);CD8insert ignore into journalissuearticles values(+); T cytotoxic cells were significantly higher insert ignore into journalissuearticles values(p<0.05); than the control group. When the patients were divided into early and advanced stages, it was observed that CD38 expression on T cells was significantly lower in advanced-stage patients insert ignore into journalissuearticles values(p< 0.05); Total lymphocyte count and CD3insert ignore into journalissuearticles values(+); T lymphocyte count were negatively correlated with the duration of hospitalization as statistically significant insert ignore into journalissuearticles values(p <0.05);. Conclusion: Our data showed that the SARS-CoV-2 primarily affects T lymphocytes. It was thought that this effect occurred by impairment of development and activation of T lymphocytes. There are some discordances among the studies on T lymphocytes in the literature.
Keywords : COVID 19, flow cytometry, lymphocytes, SARS CoV 2, T cells