Does ramelteon have an ameliorative effect in MTX-induced testicular injury?

Authors : Dilek ULUSOY KARATOPUK, Nureddin ATASOY, Duygu KUMBUL DOĞUÇ

Pages : 1034-1039

View : 44 | Download : 39

Publication Date : 2023-09-28

Article Type : Research Paper

Abstract :Aims: The aim of this study was to investigate the potential protective effect of Ramelteon insert ignore into journalissuearticles values(RMT);, which exhibits antioxidant, anti-inflammatory and antiapoptotic properties, against testicular damage induced by Methotrexate insert ignore into journalissuearticles values(MTX);, which is widely used in the treatment of various diseases, including chemotherapy. Methods: 32 Wistar albino rats were equally divided into four groups: Control insert ignore into journalissuearticles values(group I);, MTX insert ignore into journalissuearticles values(group II);, MTX+RMT insert ignore into journalissuearticles values(group III); and RMT insert ignore into journalissuearticles values(group IV);. Histologic evaluation was performed using Hematoxylin and Eosin insert ignore into journalissuearticles values(H&E); staining, immunohistochemical analysis using TNF-alpha and Cas-3, and biochemical evaluation using TAS, TOS and OSI. Results: Histologic analysis using H&E staining revealed a significant difference between group I and groups II and III insert ignore into journalissuearticles values(p 0.05);. While normal histologic structures were observed in groups I and IV, histopathologic findings were noted in groups II and III. Immunohistochemical evaluation of TNF-alpha and Cas-3 showed a significant difference between group I and groups II and III insert ignore into journalissuearticles values(p 0.05);. The highest immunostaining intensity was observed in group II. Biochemical evaluation revealed statistically significant differences in TAS, TOS and OSI parameters reflecting oxidative stress differences between the groups insert ignore into journalissuearticles values(p<0.05);. Conclusion: Anti-inflammatory, antioxidant and antiapoptotic properties of RMT demonstrated its protective effect against MTX-induced testicular injury in rats. Histological, immunohistochemical and biochemical analyses underline the potential role of RMT as a protective agent in MTX-induced testicular injury. This research aims to contribute to the understanding of potential applications to reduce the adverse effects of MTX therapy on reproductive health.
Keywords : Methotrexate, ramelteon, rat, testicular injury