Distribution of the patterns in patients with positive antinuclear antibody and anti-extractable nuclear antigen

Authors : Keramettin YANIK

Pages : 31-36

Doi:10.5455/jicm.11.20160629

View : 19 | Download : 11

Publication Date : 2016-09-30

Article Type : Research Paper

Abstract :Background: Early diagnosis of autoimmune disorders is critical in order to prevent complications, morbidity and mortality. Antinuclear antibody insert ignore into journalissuearticles values(ANA); tests are commonly used in support of the diagnosis of autoimmune diseases. In the present study, we aimed to compare and evaluate the ANA patterns which were determined via an IFA method for the detection of the presence of autoantibody against extractable nuclear antigens insert ignore into journalissuearticles values(ENA);. Material and Methods: Antinuclear antibodies were tested for a total of 5453 patients admitted to various clinics of Ondokuz Mayıs University Medical Faculty. A 1:100 dilution of each serum sample was used, and the presence of ANA and staining pattern was evaluated with ANA-IFA. A total of 616 ANA-positive samples were also tested for anti-ENA using the same method. Results: ANA tests were detected as positive in 843 samples insert ignore into journalissuearticles values(15.5%);. Amongst patients, 624 insert ignore into journalissuearticles values(74%); were female, and 219 insert ignore into journalissuearticles values(26%); were male. Anti-ENA antibodies were positive for 192 insert ignore into journalissuearticles values(31.2%); of 616 which are positive for ANA and of which anti-ENA was sought. Of those, the most common-ANA patterns were detected as speckled in 144 insert ignore into journalissuearticles values(75.0%); samples, nucleolar in 20 insert ignore into journalissuearticles values(10.4%);, and homogeneous in 13 insert ignore into journalissuearticles values(6.8%); samples. The most common anti-ENA antibodies were found to be anti-SS-A in 50 samples insert ignore into journalissuearticles values(26.0%);, RO-52 in 39 insert ignore into journalissuearticles values(20.3%);, and anti-scl-70 in 34 insert ignore into journalissuearticles values(17.7%); samples. Conclusion: Findings of the present study suggested that anti-ENA antibodies do not follow a specific ANA pattern an ANA pattern isn’t related to a specific anti-ENA antibody type. However, the presence of various ANA patterns may sometimes be associated with a specific disease or a syndrome. Detection of antinuclear antibodies, determination of the ANA patterns and, assessment of different ENA antibody types and interpretation of the results altogether will help the clinician significantly in the diagnosis of autoimmune diseases.
Keywords : Antinuclear antibodies, indirect immune fluorescence, extractable nuclear antigen