Distribution characteristics of RIG-I receptors of innate immunity in experimental diabetes mellitus and administration of nonspecific blockers of TNFα.

Authors : Anna DEGEN

Pages : 50-59

View : 14 | Download : 13

Publication Date : 2018-09-30

Article Type : Research Paper

Abstract :Background: We study type 1 diabetes as examples in which interactions between host and viruses have been implicated in autoimmune pathology. The RIG-I-like receptors insert ignore into journalissuearticles values(RLRs); as a sensors of RNA virus infection which can initiate and modulate antiviral immunity have been studied. The aim of the study was to determine the features of expression of retinoic acid-inducible gene-I insert ignore into journalissuearticles values(RIG-I); receptors in GALT in experimental diabetes mellitus insert ignore into journalissuearticles values(EDM); and after administration of pentoxifylline. Materials and Methods: To determine structure of population of RIG +-cells we used the analysis of serial histological sections using the method of indirect immunofluorescense with monoclonal antibodies to RIG-I of rat insert ignore into journalissuearticles values(Santa Cruz Biotechnology, USA);. Results: It has been established that diabetes development was accompanied by an increase in total density RIG+ cells, population density of RIG+ macrophages and increase the concentration of the RIG protein in these cells in the lymphoid structures of ileum at 2nd week. But this data showed a dynamics to decrease to control values by the 4th week of disease. Pentoxifylline insert ignore into journalissuearticles values(PTX); administration of diabetic animals resulted in a decrease of the total density RIG+ cells, population density RIG+ dendritic cells and RIG+ lymphocytes on the at 2nd week of pathology, and on the 4th week of the disease this data showed dynamics to an increase. Conclusions: All of these show that expression of RIG-I in ileum immunopositive cells can influence the differentiation of immunopositive cells and their ability to produce pro-inflammatory cytokines, thus acting as one of triggers of diabetes development and progression.
Keywords : type 1 diabetes mellitus, RIG like receptors, retinoic acid inducible gene I