The antibacterial effects of vitamin D3 against mutans streptococci: an in vitro study

Authors : Manal Mohamed Mansour ALMOUDİ, Mohamed Ibrahim ABU HASSAN, Hassanain ALTALİB, Hasnah Begum Said Gulam KHAN, Siti Arisya Binti NAZLİ, Nur Aina Efira Binti EFFANDY

Pages : 8-15

View : 28 | Download : 10

Publication Date : 2021-01-30

Article Type : Research Paper

Abstract :Purpose: This study aims to evaluate the antimicrobial effects of the cholecalciferol vitamin D3 against Streptococcus sobrinus insert ignore into journalissuearticles values(Strep. sobrinus); and Streptococcus mutans insert ignore into journalissuearticles values(Strep. mutans); bacteria in vitro that is considered the main causative bacteria in dental caries development. Materials and Methods: The antimicrobial effects of vitamin D3 were evaluated against Strep. sobrinus and Strep mutans using the agar disc diffusion method. The minimum inhibitory concentration insert ignore into journalissuearticles values(MIC); and minimum bactericidal concentration insert ignore into journalissuearticles values(MBC); of vitamin D3 were determined using a microdilution method following the guidelines by the Clinical Laboratory Standards Institute insert ignore into journalissuearticles values(CLSI);. Scanning electron microscope insert ignore into journalissuearticles values(SEM); was used to evaluate the morphological changes of bacterial cells following exposure to vitamin D3. Results: Strep. sobrinus was more sensitive to vitamin D3 compared to Strep. mutans bacteria. The MIC values of vitamin D3 against Strep. sobrinus and Strep. mutans were 60 µg/mL and 250 µg/mL respectively whereas the MBC values were 120 µg/mL and 500 µg/mL, respectively. Moreover, significant changes in the bacterial morphology were observed in treated bacterial cells with vitamin D3 as compared to the untreated control bacteria using SEM. Conclusion: These findings suggested that vitamin D3 has excellent antimicrobial effects against Strep. sobrinus and Strep. mutans and may be considered as a promising compound in the prevention of dental caries in the future. Further research is recommended to elucidate the mechanism of vitamin D3 on these bacteria.
Keywords : Vitamin D3, Cholecalciferol, Streptococcus sobrinus, Streptococcus mutans, antibacterial effect