Influence of subinhibitory-concentration (sub-MIC) Cefetoxime on biofilm formation. SEM study of ESBL-producing Salmonella typhi

Authors : Rahul NARASANNA

Pages : 67-74

Doi:10.5799/jmid.328786

View : 8 | Download : 3

Publication Date : 2017-06-01

Article Type : Research Paper

Abstract :Objectives: In the present study, we have analyzed ESBL-producing S. typhi’s capability in forming a significant amount of biofilm on plastic and glass surface, and the influence of cefetoxime on biofilm development at subinhibitory insert ignore into journalissuearticles values(Sub-MIC); concentration. Methods: Nine strains of cefetoxime-mediated ESBL-producing S. typhi were used in the study. S. typhi formed biofilm on plastic and glass materials; it was demonstrated using micro titre plate insert ignore into journalissuearticles values(MTP); and standard test tube methods. Comparative study of the influence of cefetoxime on biofilm formation in its MIC insert ignore into journalissuearticles values(128 µg/ml); and at sub-MIC insert ignore into journalissuearticles values(64 µg/ml); was demonstrated by microtitre plate method. The biofilm production was observed in SEM images, statistical analysis insert ignore into journalissuearticles values(ANOVA); showed significant increase in cell surface and volume due to the influence of Cefetoxime. Results: Of the nine selected isolates, two S. typhi strains, namely BST 51 and BST 130, produced relatively strong biofilm in the presence of cefetoxime at sub-MIC level insert ignore into journalissuearticles values(64 µg/ml);, comparatively weak biofilm formation at MIC level insert ignore into journalissuearticles values(128 µg/ml);. Typical morphological changes were observed in cefetoxime-resistant strains, S. typhi BST 51 and BST 130, in comparison to cefetoxime-sensitive strain S. typhi BST 63 used as a control. We found an increase in surface and volume of a cell in response to cefetoxime and statistical data insert ignore into journalissuearticles values(ANOVA); proved that resistant strains were significantly different from control strains. Conclusion: The above study clearly shows that cefetoxime at sub-MIC level efficiently induces biofilm formation and promotes changes in morphology of the cell. J Microbiol Infect Dis 2017; 7insert ignore into journalissuearticles values(2);: 67-75 
Keywords : Salmonella typhi, Biofilm, Cefetoxime, Subinhibitory concentration Sub MIC, MIC, SEM