Prevalence and molecular characterization of extended spectrum beta-Lactamases-producing uropathogenic Escherichia coli isolated in Zakho, Iraq

Authors : Reem F Polse, Samira Y Yousif, MAHDE SALEH ASSAFİ

Pages : 163-167

Doi:10.5799/jmid.328863

View : 10 | Download : 7

Publication Date : 2016-12-01

Article Type : Research Paper

Abstract :Objectives: Uropathogenic Escherichia coli are the most important group of microorganisms responsible for urinary tract infection. A high percentage of uropathogenic E. coli over the world are detected to be ESBLs producers, which is now a problem that limits therapeutic options. The aim of this study is to determine the prevalence of ESBLs E. coli and study the prevalence of different ESBLs genotype patterns among the ESBLs producing isolates. Methods: One hundred and six uropathogenic E. coli isolates were analyzed for their ESBL production by molecular methods. Results: 55 insert ignore into journalissuearticles values(52%); isolates were detected as extended spectrum β-lactamases insert ignore into journalissuearticles values(ESBLs); producers. Based on the PCR results, all E. coli isolates possessed one or more ESBL gene. CTX-M type ESBL was the most dominant ESBL insert ignore into journalissuearticles values(87.2%); among the isolates. While those for TEM-type and SHV-type were 54.5% and 21.8% respectively. Six genotype patterns were detected insert ignore into journalissuearticles values(TEM, CTX-M, TEM+SHV, TEM+CTX-M, SHV+CTX-M and TEM+SHV+CTX-M);. The genotype CTX-M was the most prevalent genotype insert ignore into journalissuearticles values(40%); followed by the genotype TEM+CTX-M combination insert ignore into journalissuearticles values(30.9%);. The occurrences of the genotypes insert ignore into journalissuearticles values(TEM, TEM+SHV, SHV+CTX-M and TEM+SHV+CTX-M); were 7.3%, 5.5%, 5.4%, 10.9%, respectively. Conclusions: Control measures and education programs are necessary to avoid the uncontrollable use of β-lactam and cephalosporins in order to minimize the emergence of ESBLs producing E. coli. J Microbiol Infect Dis 2016;6insert ignore into journalissuearticles values(4);: 163-167  
Keywords : ESBL, Uropathogenic E coli, SHV, TEM, CTX M, Iraq