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  • Journal of Microbiology and Infectious Diseases
  • Volume:6 Issue:4
  • Group B streptococcus detection in China: comparison of different screening methods and different sa...

Group B streptococcus detection in China: comparison of different screening methods and different sampling sites

Authors : İ Wang, Yingna Song, Liangkun Ma, JUNTAO LİU, YİNGCHUN XU, JİE Yİ
Pages : 179-183
Doi:10.5799/jmid.vi.328930
View : 15 | Download : 10
Publication Date : 2016-12-01
Article Type : Research Paper
Abstract :Objectives: To evaluate the use of real-time polymerase chain reaction insert ignore into journalissuearticles values(RT-PCR); and bacterial culture methods to detect group B streptococcus insert ignore into journalissuearticles values(GBS); in Chinese pregnant women in the third trimester; to separately assess the prevalence of rectal and vaginal GBS colonization ; and to determine the antimicrobial susceptibility pattern of the isolates. Methodology: Samples were collected from 505 women at 35 and 37 weeks gestation at the Peking Union Medical College Hospital. Bacterial culture and RT-PCR were performed. Antimicrobial susceptibility to commonly used antibiotics was also analyzed. Results: The overall GBS colonization rate was 7.5%. The colonization rate, sensitivity, and negative predictive value of the bacterial culture method were 2.8%, 36.8%, and 95.1%, respectively, and these values were 7.3%, 97.4%, and 99.8%, respectively, for PCR insert ignore into journalissuearticles values(p<0.001);. The GBS colonization rate of the rectum insert ignore into journalissuearticles values(6.7%); was higher than that of the vagina insert ignore into journalissuearticles values(2.8%); insert ignore into journalissuearticles values(p=0.005);. Antimicrobial susceptibility testing showed that 100% were sensitive to penicillin, cephalosporin and vancomycin. Conclusions: RT-PCR was found to be a rapid and sensitive test for the detection of GBS colonization in Chinese pregnant women. Rectal swabbing was also important for detecting GBS colonization. β-lactams are the first-line antibiotics used for the treatment of GBS. J Microbiol Infect Dis 2016;6insert ignore into journalissuearticles values(4);: 179-183  Group B streptococcus, Real-time polymerase chain reaction, Sampling sites,
Keywords : Group B streptococcus, Real time polymerase chain reaction, Sampling sites

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