Nuclear Matrix Protein 22 and Cytological Findings in Urine of Residents of Urogenital Schistosomiasis in an Endemic Community

Authors : Ukeme Gideon ETUK, Paul Columba INYANGETOH, Mfonisa İniobong UDONKANG, Emmanuel Monday Japhet, Uloma OparaOsuoha

Pages : 89-96

Doi:10.5799/jmid.458454

View : 8 | Download : 3

Publication Date : 2018-09-15

Article Type : Research Paper

Abstract :Objective: Urogenital schistosomiasis has continued to cause severe morbidity among residents of Adim community. The aim of the study was to screen both adults and children residents of this community for urine tumour markers and to stain cytologically for epithelial changes which indicate the risk of them being predispose to bladder cancer. Methods: Terminal urine samples were collected by simple random sampling method from 350 subjects insert ignore into journalissuearticles values(300 children and 50 adults); in Adim Community for microscopy using standard parasitological methods, urinalysis, ELISA-based Nuclear Matrix Protein 22 insert ignore into journalissuearticles values(NMP22);, and Papanicolaou- and Alcian blue-stained cytological analysis. Results: S. haematobium infection was observed in 18 insert ignore into journalissuearticles values(5.1%);, proteinuria 21 insert ignore into journalissuearticles values(6.0%); and hematuria 30 insert ignore into journalissuearticles values(8.6%);. Age group 25-31 years had the highest level of NMP22 values insert ignore into journalissuearticles values(1692.3 ± 46.4); among the adults and children. The difference was statistically significant insert ignore into journalissuearticles values(P=0.001);. In general male subjects had higher NMP22 values insert ignore into journalissuearticles values(1214.6+ 487.1); than females insert ignore into journalissuearticles values(1198.9+ 472.9); insert ignore into journalissuearticles values(P=0.389);. The cytological findings showed abnormal epithelial cells in Papanicolaou stain 2 insert ignore into journalissuearticles values(0.57%); and hyaluronic acid 1 insert ignore into journalissuearticles values(0.29%);. Cytological findings with Papanicolaou stain revealed metaplasia 1 insert ignore into journalissuearticles values(0.3%);, mild dysplasia 1 insert ignore into journalissuearticles values(0.3%);, inflammation 6 insert ignore into journalissuearticles values(1.7%);, and hyaluronic acid positive cells 1 insert ignore into journalissuearticles values(0.29%);. Conclusion: The study showed S. haematobium prevalence of 5.1% but NMP 22 values were not positive for bladder cancer. The cytological findings revealed metaplasia, mild dysplasia and hyaluronic acid positive epithelial changes which are indicators for bladder cancer. J Microbiol Infect Dis 2018; 8insert ignore into journalissuearticles values(3);:89-96
Keywords : Bladder cancer, Cytology, Nuclear Matrix Protein 22, Urogenital Schistosomiasis