Molecular Epidemiology of Multidrug-resistant Escherichia coli from Urinary Tract Infections

Authors : Divya SUKUMARAN, Mohamed HATHA

Pages : 66-73

Doi:10.5799/jmid.951495

View : 8 | Download : 3

Publication Date : 2021-06-15

Article Type : Research Paper

Abstract :Objectives: The purpose of this study was to investigate the phylogenetic groups, antibiotic resistance, antibiotic resistance genes insert ignore into journalissuearticles values(ARGs);, integrons, extraintestinal virulence genes and genetic diversity of Escherichia coli isolates from human urinary tract infection. Methods: A total of 100 E. coli isolates were collected from patients with urinary tract infections in Kerala, South India. Antibiotic susceptibility testing of all E. coli isolates against different antibiotics was determined by the disc diffusion method. Phylogenetic groups, extraintestinal virulence genes, ARGs, and integrons were detected by PCR. Enterobacterial repetitive intergenic consensus polymerase chain reaction insert ignore into journalissuearticles values(ERIC-PCR); was used to check the genetic relatedness among E. coli isolates. Results: E. coli isolates have mainly belonged to phylogenetic group B2. Resistance to ampicillin was most frequent among the E. coli isolates followed by resistance to cefoxitin, cefpodoxime, nalidixic acid, trimethoprim, and co-trimoxazole. Among E. coli isolates, 96% were multidrug-resistant insert ignore into journalissuearticles values(MDR);, and 86% and 32% harbored ARGs and integrase 1 insert ignore into journalissuearticles values(int1); respectively. Seventy-nine percent of the isolates were extraintestinal pathogenic E. coli insert ignore into journalissuearticles values(ExPEC);, and 86% of them insert ignore into journalissuearticles values(n = 68); harbored ARGs. One extensively drug-resistant insert ignore into journalissuearticles values(XDR); ExPEC was obtained in this study. The present study revealed a significant association between the presence of virulence genes and antibiotic resistance. A high degree of genetic diversity was observed among the ARGs-harboring E. coli isolates. Conclusion: Understanding the association between extraintestinal virulence genes and antibiotic resistance genes would result in the proper treatment of urinary tract infections. J Microbiol Infect Dis 2021; 11insert ignore into journalissuearticles values(2);:66-73.
Keywords : antibiotic resistance, ExPEC, India, Escherichia coli,