Lactobacillus acidophilus Supplementation Restores Gut Epithelial Integrities and Barrier Functions in Non-specific Diarrhea

Authors : Hossain Ferdaus Mohd ALTAF, Sumaiya Islam FARZANA, Khan PARVEZ, Zahran EMAN, Atif Shaikh MUHAMMAD, Uddin Md NAZIM, Zinnah Kazi Mohammad ALİ, Khan IMDAD ULLAH, Rahman Md MASUDUR

Pages : 54-62

Doi:10.5799/jmid.1130109

View : 8 | Download : 2

Publication Date : 2022-06-15

Article Type : Research Paper

Abstract :Objectives: The study was aimed to evaluate the roles of Lactobacillus acidophilus supplementation in maintaining intestinal epithelial integrities, tight junction proteins, and adhesion molecules in non-specific diarrhea. Methods: In this study, we used the pre-weaned BL/6 pups insert ignore into journalissuearticles values(3 weeks of age, same litters); as a model animal. We supplied the non-sterilized and poor-quality water to experimental pups insert ignore into journalissuearticles values(n=7); to develop non-specific diarrheic symptoms. Then diarrheic pups were supplemented with Lactobacillus acidophilus insert ignore into journalissuearticles values(LA); for three consecutive weeks. The control group insert ignore into journalissuearticles values(n=5); was supplied with sterilized water and no LA. The sampling and analysis were performed on day 0, day 7, day 14, and day 21. The expressions of pro-inflammatory cytokines insert ignore into journalissuearticles values(IL-6, TNF-a); and tight junction proteins insert ignore into journalissuearticles values(TJPs); of gut mucosa were determined using qRT-PCR. And the serum cytokines level was screened through sandwich ELISA. Results: The intestinal cytoskeletal integrity becomes disrupted and characterized by lower ZO-1, Occludin, Claudin-1, Claudin-5, and JAM mRNA expressions upon real-time qRT-PCR. However, Claudin-4 was found to be not affected and illustrated with a higher expression like control pups. Interestingly, supplementing Lactobacillus acidophilus was found to maintain gut integrity and effectively reduce diarrheic symptoms. Like the control pups, the Lactobacillus acidophilus supplemented pups exhibited a higher expression of gut epithelial TJPs and adhesion molecules. Moreover, the diseased pups produced significantly increased IL-6, and TNF-α production in blood serum, compared to control BL/6 pups. Conclusion: We concluded that L. acidophilus supplementation might orchestrate the equilibrium of gut health and immunity against non-specific diarrhea.
Keywords : mice, gut immunity, tight junctions, and adhesion molecules, Lactobacillus acidophilus supplementatio