Evaluating The Effectiveness of A Non-Locally Developed Commercial Phage Cocktail on Kenyan Pseudomonas aeruginosa Isolates

Authors : Ivy Jepkurui MUTAIL, Martin GEORGES, Atunga NYACHIEO, Eric ODOYO, Anthony Kebira NYAMACHE, Jillani NGALLA

Pages : 25-30

Doi:10.5799/jmid.1265378

View : 9 | Download : 1

Publication Date : 2023-03-15

Article Type : Research Paper

Abstract :Objectives: The use of bacteriophages insert ignore into journalissuearticles values(phages); as an alternative treatment for multidrug-resistant bacteria has recently gained popularity. Phage cocktails have been proposed for broad-spectrum therapeutic effects against such resistant bacteria. However, the effectiveness of non-locally formulated phage cocktails for therapy on Kenyan isolates has yet to be evaluated and is a subject of investigation. This study aimed to determine the in vitro effectiveness of a non-locally made commercial pyophage cocktail on clinical Pseudomonas aeruginosa isolates from Kenya. Methodology: Forty-nine P. aeruginosa isolates from Kenya were subjected to a pyophage cocktail for efficacy studies using direct spot test insert ignore into journalissuearticles values(DST); and efficiency of plating insert ignore into journalissuearticles values(EOP);. Results: The success rate of the cocktail was observed on 16.3% insert ignore into journalissuearticles values(8/49); isolates only and ineffective on 83.7% insert ignore into journalissuearticles values(41/49); isolates. Six of the eight isolates that showed cross-reactivity from DST had complete lysis with a faintly hazy background. Five of these six isolates resulted in successful and high phage progeny production in plaquing efficiency insert ignore into journalissuearticles values(EOP ≥ 0.5);. Conclusion: Non-locally made commercial pyophage cocktail was ineffective against the 83.7% endemic clinical strains of the Kenyan P. aeruginosa isolates, demonstrating the importance of locally derived phage cocktails against endemic and multidrug-resistant isolates. J Microbiol Infect Dis 2023; 12insert ignore into journalissuearticles values(4);:25-30.
Keywords : Pyophage cocktail, P aeruginosa, spot test, the efficiency of plating, host range