The Molecular Characterization and Evolutionary History Analysis of Cotton leaf curl Multan virus (CLCUMUV) Infecting (Hibiscus sabidarrifa, Hibiscus rosa-sinensis) in Punjab Province of Pakistan

Authors : Muhammad ARİF

Pages : 18-30

Doi:10.58728/joinabt.1301944

View : 33 | Download : 35

Publication Date : 2023-06-30

Article Type : Research Paper

Abstract :The plants insert ignore into journalissuearticles values(Hibiscus sabidarrifa and Hibiscus rosa-sinensis); are cultivated in many tropical and subtropical climates for their versatile usage. Plant viruses have a significant capacity to cause the maximum losses in agricultural production. It is assumed that these viruses are responsible for the emergence of about half of plant diseases which are a major threat to food safety or ecological integrity worldwide. Geminiviruses are family of plant viruses with characteristic twinned quasi-icosahedral virions and a small, single-stranded circular DNA genome. Some plants insert ignore into journalissuearticles values(H. sabidarrifa and H. rosasinensis); showing the characteristic symptoms of viruses were observed. The presence of whitefly vector presumed that these plants might have begomovirus. A 550 bp of amplicon size as a plasmid was obtained from the infected plants after PCR reaction. The obtained amplicon was amplified with sequence specific primers to get the full length genome. The sequence analysis has proved the existence of Cotton leaf curl Multan virus insert ignore into journalissuearticles values(CLCUMUV); from symptomatic plants. The full genome sequence analysis revealed a 2.4-2.5 kb full genome of CLCUMUV infecting Roselle species. A phylogenetic and evolutionary history analysis has confirmed the association of this virus with other aligned viruses. Overall, we have identified one new species as host of begomovirus in this area.
Keywords : Roselle, Begomovirus, AC2 geni, Filogenetik analiz, evrimsel tarih, nükleotit karşılaştırması