Algerian Bunium incrassatum Seeds: Effects of Extraction Solvent Polarity on Phenolic Profile and Antioxidant Activity

Authors : Fethi TOUL, Amina DJENDAR, Meryem SELADJİ, Farid BERROUKECHE

Pages : 415-422

Doi:10.18596/jotcsa.1058060

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Publication Date : 2022-05-31

Article Type : Research Paper

Abstract :Bunium incrassatum, vernacularly called ‘Talghouda’ is one of the most important plant species of the traditional Algerian pharmacopeia, used for medicinal and culinary purposes. Despite of its benefits, it still remains a scientifically neglected species, especially with regards to the phytochemical profile. The present study aims to evaluate the antioxidant potentials of Bunium incrassatum seeds’ extracts prepared in different solvents with increasing polarities. The antioxidant potentials of extracts were measured using DPPH and β-carotene Bleaching assays. Total phenolic, flavonoid, and tannin contents were also spectrophotometrically determined. The phenolic profiles were screened through RP-HPLC. Methanol extract showed the highest DPPH scavenging potential and also the highest inhibitory potential against β-carotene bleaching insert ignore into journalissuearticles values(IC50=0.15±0.02 and 0.41±0.03 mg/mL, respectively);. Thirteen phenolic compounds were identified, a flavanol insert ignore into journalissuearticles values(catechin);, two flavonols insert ignore into journalissuearticles values(kaempferol, quercetin);, two flavanones insert ignore into journalissuearticles values(hesperetin, naringenin); and eight phenolic acids: caffeic acid, chlorogenic acid, ellagic acid, ferulic acid, gallic acid, p-coumaric acid, sinapic acid, and syringic acid. These results support the few previous studies showing that Bunium incrassatum, as an endemic species, is considered as a good source of bioactive compounds that requires further investigations.
Keywords : Bunium incrassatum, seeds, phenolic compounds, antioxidant potential, RP HPLC