Eco-friendly Approach for Silver Nanoparticles Synthesis from Lemon Extract and their Anti-oxidant, Anti-bacterial, and Anti-cancer Activities

Authors : Nisreen Jassam ALAALLAH, Ekhlas ABD ALKAREEM, Aseel GHAİDAN, Nuha A IMRAN

Pages : 205-216

Doi:10.18596/jotcsa.1159851

View : 13 | Download : 23

Publication Date : 2023-02-28

Article Type : Research Paper

Abstract :To create silver nanoparticles, researchers use bionanotechnology techniques because they are economical and environmentally friendly. The current study shows that lemon juice insert ignore into journalissuearticles values(Citrus-limon); can be used to biosynthesize silver nanoparticles insert ignore into journalissuearticles values(Ag NPs);. The synthesized silver nanoparticles have been characterized by the surface plasmon resonance insert ignore into journalissuearticles values(SPR); measured at λmax = 430 nm, confirming the formation of AgNPs. Moreover, Fourier Transform Infrared insert ignore into journalissuearticles values(FTIR); analysis was carried out to identify possible bio-molecules responsible for the bio-reduction of silver ions. The x-ray diffraction insert ignore into journalissuearticles values(XRD); peaks at insert ignore into journalissuearticles values(111, 200, 220, 222, and 311); confirm the found face-centered cubic insert ignore into journalissuearticles values(FCC); crystal structure of AgNPs in solution. Transmission-Electron-Microscopy insert ignore into journalissuearticles values(TEM); images showed that AgNPs have spherical morphology with sizes ranging from 10-50 nm. Furthermore, the Particles Size Analyzer insert ignore into journalissuearticles values(PSA); confirmed these sizes and ranges. Synthesized AgNPs have high anti-oxidant activity according to the insert ignore into journalissuearticles values(scavenging of DPPH radicals, total anti-oxidant, and reducing power); assays. Also, the anti-bacterial activity of AgNPs was evaluated by a well diffusion method, and the results suggest that they are more sensitive to gram-positive bacteria than gram-negative ones, with the average diameter of the inhibition zones for AgNPs ranging from 4.11 to 25.87 mm and 1.38 to 22.3 mm against S. aureus and E. coli bacteria, respectively. In vitro studies of AgNPs against MCF-7 breast cancer cell s lines showed a good cytotoxic effect p<0.05 with an IC50 value of 47 μg/mL; this study could be beneficial for nanotechnology-based pharmaceutical and biomedical applications.    
Keywords : Silver nano particles, Citrus limon extract, Anti oxidant activity, Anti bacterial activity, Anti cancer activity