INVESTIGATION OF BIOLOGICAL ACTIVITIES OF 4-HYDROXY-3-(2-HYDROXY-5-METHYLBENZYLIDEAMINO) BENZENESULPHONIC ACID

Authors : Elif ÜNALDI, Neslihan DEMİR

Pages : 379-388

Doi:10.26900/jsp.3.038

View : 5 | Download : 3

Publication Date : 2019-10-29

Article Type : Research Paper

Abstract :In this study, the antimicrobial activity, DNA cleavage, DNA binding and antioxidant properties of a sulfonic acid-based imine compound were investigated. The antimicrobial activity of the compound was investigated for minimum inhibitory concentration insert ignore into journalissuearticles values(MIC); against  some bacteria and yeast cultures.  The DNA cleavage activity of the compound was investigated as hydrolytic and oxidative with the gel electrophoresis method. H2O2 was used as an oxidizing agent for detection of the cleavage activity mechanism. The Ultraviolet-Visible insert ignore into journalissuearticles values(UV-Vis); field absorption spectroscopy method was used to determine the binding effect to DNA. The sulfonic acid-based imine compound reacted with Calf Thymus DNA insert ignore into journalissuearticles values(CT-DNA); which was examined by UV-Vis absorption spectroscopy. The free radical scavenging activity was determined using the 2,2-Diphenyl-1-picrylhydrazyl insert ignore into journalissuearticles values(DPPH); method. The studied compound was found to be effective on yeast and bacteria at different concentrations. The compound was found to be more effective on Staphylococcus aureus ATCC 25923 and Enterococcus faecalis ATCC 29212 bacteria. DNA cleavage study showed that the compound cleaved DNA without any external agents in hydrolytic and oxidative manner. UV-Vis spectroscopy studies of the interactions between the compound and CT-DNA showed that the compound interacts with CT-DNA via electrostatic binding. The compound to be tested was compared with the butylated hydroxytoluene insert ignore into journalissuearticles values(BHT); solution used as standard. It was found that the compound exhibits good antioxidant activity.
Keywords : Sulfonic acid, antimicrobial activity, antioxidant activity, DNA cleavage, DNA binding