ELECTROCHEMICAL BIOSENSOR FOR BRCA1 GENE AND TAMOXIFEN INTERACTION

Authors : Suzan YANIK, Dilsat OZKANARIKSOYSAL, Selehattin YILMAZ

Pages : 35-48

Doi:10.26900/jsp.4.004

View : 12 | Download : 5

Publication Date : 2020-01-31

Article Type : Research Paper

Abstract :The electrochemical nanobiosensor was designed for the determination of specific DNA sequences related to breast cancer 1 insert ignore into journalissuearticles values(BRCA1); gene and interaction between Anticancer Drug Tamoxifen insert ignore into journalissuearticles values(TAM); and related DNA sequences by using pencil graphite electrode insert ignore into journalissuearticles values(PGE);, bare and multi-walled carbon nanotube insert ignore into journalissuearticles values(MWCNT); contained screen printed carbon electrodes insert ignore into journalissuearticles values(SPEs); for the first time. Here, biomolecular interaction between TAM and DNA was investigated differential pulse voltammetry insert ignore into journalissuearticles values(DPV); based on not only guanine signal but also TAM oxidation response. It was obtained that the guanine signal at about +1.00V obtained from probe DNA or hybrid DNA shows a remerkable increase after the interaction with TAM. Additionally, it was found that TAM interact with guanine bases and TAM signal which is near the guanine oxidation area also increase after the interaction with DNA. Consequently, the prepared biosensor offer suitable platform for the analysis of DNA hybridization and TAM-DNA interaction sensitively.
Keywords : Tamoxifen, BRCA1, Electrochemical DNA nanobiosensor, carbon nanotube, Differential Pulse Voltammetry, Drug DNA interaction, Pencil Graphite Electrode, Screen Printed Electrode