The Combined Effects of Etanercept Plus Methylprednisolone on Pancreatic Oxidative Stress in Acute Pancreatitis Induced by Cerulein

Authors : Murat GÜNAY, Erol KILIÇ, Ramazan AMANVERMEZ

Pages : 12-16

View : 38 | Download : 9

Publication Date : 2012-02-15

Article Type : Research Paper

Abstract :ABSTRACT Whether etanercept plus metylprednisolone reduce pancreatic oxidative stress and injury during pancreatitis is unknown well. The aim of this study was to examine the therapeutic effects of etanercept and methylprednisolone insert ignore into journalissuearticles values(MP); on acute pancreatitis and oxidative stress in acute pancreatitis insert ignore into journalissuearticles values(AP); induced by cerulein in a rat model. The study was performed with 48 rats divided into 6 groups insert ignore into journalissuearticles values(n = 8/group);: 1-sham, 2-cerulein-induced pancreatitis insert ignore into journalissuearticles values(over 10 hours);, 3- cerulein-pancreatitis insert ignore into journalissuearticles values(over 20 hours);, 4-etanercept insert ignore into journalissuearticles values(5 mg/kg, i.p.);, 5-methylprednisolone insert ignore into journalissuearticles values(10 mg/kg, i.m.);, 6-etanercept plus methylprednisolone. Also, the rats in groups 4, 5, and 6 were cerulein-induced pancreatitis at 20 hours. After the treatment, the pancreas and blood were taken for histopathological and biochemical analysis. All cerulein-treated rats developed biochemical and pathological acute pancreatitis after 10-20 hours. The markers of oxidative stress such as protein carbonyls, lipid peroxidation, and myeloperoxidase insert ignore into journalissuearticles values(mpo); in the pancreas tissues were increased in the group 2 and 3, but these were lower in etanercept and MP-treated rats compared to groups 3. Pancreatic mpo activity was considerably reduced in pancreas tissues at 20 h after the administration of etanercept plus metylprednisolone in the group 6 compared to group 3. In AP induced by cerulein, the treatment of etanercept plus methylprednisolone may ameliorate pancreatic oxidative stress in rats. 
Keywords : Etanercept, methylprednisolone, acute pancreatitis, oxidative stress

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