Effects of experimental diabetes on testis proliferations and apoptosis in rats

Authors : Cevat AKTAS, Mehmet KANTER, Mustafa ERBOĞA, Hüseyin TİMURKAN

Pages : 94-98

Doi:10.5835/jecm.v28i3.1009001881

View : 52 | Download : 10

Publication Date : 2012-02-09

Article Type : Other Papers

Abstract :ABSTRACT This work aimed to investigate the role of diabetes on cell proliferation, and apoptosis in testis. The rats were randomly allotted into one of two experimental groups: control and diabetic group; each group contain 10 animals. Diabetes was induced by a single intra¬peritoneal injection of STZ insert ignore into journalissuearticles values(50 mg/kg);. Testicular damage was examined by using he¬matoxylin and eosin, immunohistochemical staining of proliferating cell nuclear antigen insert ignore into journalissuearticles values(PCNA);, and apoptosis was determined by terminal-deoxynucleotidyl-transferase medi¬ated dUTP nick end labeling insert ignore into journalissuearticles values(TUNEL);. Potential disorders associated with seminiferous tubular sperm formation were evaluated using the Johnsen score. The mean seminiferous tubule diameter insert ignore into journalissuearticles values(MSTD); and mean testicular biopsy score insert ignore into journalissuearticles values(MTBS); values were signifi¬cantly decreased in diabetic group was compared to the control group. Our data indicate a significant reduction in the expression of PCNA and an enhancement in the activity of TUNEL in testis tissues of the diabetic group. The effects of diabetes on spermatogenesis can be clearly detected as a testicular cell death and decrease in MTBS, MSTD, and PCNA expression. J. Exp. Clin. Med., 2011; 28:94-98
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