The effect of prenatal exposure to 900-megahertz electromagnetic field on the newborn rat testicles

Authors : Hatice HANCI, Ersan ODACI, Haydar KAYA, Yüksel ALİYAZICIOĞLU, İbrahim TURAN, Selim DEMİR, Serdar ÇOLAKOĞLU

Pages : 0-0

Doi:10.5835/jecm.omu.30.03.019

View : 36 | Download : 12

Publication Date : 2013-11-05

Article Type : Research Paper

Abstract :Intensive use in daily life of devices with an electromagnetic field insert ignore into journalissuearticles values(EMF);, such as radios, televisions, computers and mobile phones, results in constant exposure. The fact that more than 80% of people in many countries use mobile phones has further increased concerns about the effect of EMFs. The aim of this study was to investigate the effect of exposure to a 900-megahertz insert ignore into journalissuearticles values(MHz); EMF in the prenatal term on the newborn rat testicles. Six pregnant, female Sprague Dawley rats were equally divided into control insert ignore into journalissuearticles values(CG); and EMF insert ignore into journalissuearticles values(EMFG); groups. No procedure was performed on CG. EMFG was exposed to a 900 MHz EMF for 1 h during days 13-21 of pregnancy at the same hour every day. No procedure was performed on newborn female rats after birth, and the study continued with newborn male rats. Newborn CG insert ignore into journalissuearticles values(NCG, n: 10); male rats were obtained from mothers in the CG, and newborn EMFG insert ignore into journalissuearticles values(NEMFG, n: 10); male rats from mothers in the EMFG. All animals were sacrificed and the testicles extracted at postnatal day 21. Testicular tissue samples were evaluated histopathologically under light microscope. The TUNEL technique was used to determine apoptosis in testicles. Lipid peroxidation and DNA oxidation levels were evaluated biochemically. Histopathologically, NEMFG male rats exhibited irregularities in the seminiferous tubule basal membrane and epithelium of seminiferous tubules, immature germ cells in the lumen, and a decreased diameter of seminiferous tubules and thickness of epithelium. Apoptotic index, lipid per oxidation and DNA oxidation were higher in NEMFG male rats than in NCG. Our results showed that the newborn rat testicles exposed to 900 MHz EMF in the prenatal period has been adversely affected.
Keywords : 900 Mhz Electromagnetic field, Lipid peroxidation, Newborn rat testicle, Seminiferous tubules

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