Fatty acid-binding protein 2 Ala54Thr polymorphism among psoriatic patients in Çanakkale, Turkey

Authors : Meliha Merve HIZ, Cüneyt AKI, Zerrin ÖĞRETMEN
Pages : 0-0
Doi:10.5835/jecm.v2i31.5000068738
View : 30 | Download : 8
Publication Date : 2014-09-10
Article Type : Other Papers
Abstract :A single nucleotide polymorphism insert ignore into journalissuearticles values(G-to-A); in codon 54 of exon two resulting in an alanine to threonine substitution and we hypothesized that, in psoriatic patients Ala54Thr carriers would have increased risk for psoriasis than Ala54-homozygous counterparts. 141 psoriatic patients and 131 healthy individuals who gave informed consent, were enrolled to study. FABP2 polymorphism was evaluated with melting curve analysis insert ignore into journalissuearticles values(RealTime PCR);. Genotype frequencies were estimated by gene counting. In consistency of genotype frequencies with the Hardy-Weinberg equilibrium was tested using a Chi- Square. Odds ratio insert ignore into journalissuearticles values(OR); and 95% confidence interval were calculated to estimate the risks related to insert ignore into journalissuearticles values(FABP2); Ala54Thr polymorphism. To calculate the significance of OR, Fisher’s exact test was used. Eighteen patients insert ignore into journalissuearticles values(12.8%); had Ala54/Ala54 insert ignore into journalissuearticles values(wild group); and 118 insert ignore into journalissuearticles values(83.7%); patients a mutant genotype, Ala54/Thr54 insert ignore into journalissuearticles values(55 patients, 39%); or Thr54/Thr54 insert ignore into journalissuearticles values(63 patients, 44.7%);. In control group, ten subjects insert ignore into journalissuearticles values(7.6%); had the genotype Ala54/Ala54 insert ignore into journalissuearticles values(wild-type group); and 121 insert ignore into journalissuearticles values(92.4%); participants had the genotype Ala54/Thr54 insert ignore into journalissuearticles values(n=47, 35.9%); or Thr54/Thr54 insert ignore into journalissuearticles values(n=74, 56.5%);. The genotype distribution were consistent with Hardy-Weinberg equilibrium for psoriatic and healthy groups insert ignore into journalissuearticles values(χ²calculated: 0.07 2 nd  degree);.When psoriatic patients with homozygote “AA” or heterozygote genotype “GA” were compared with the ones with normal genotype, no statistical correlation was found insert ignore into journalissuearticles values(ORAAvs GG:0.47; 95%CI: 0.20-1.09; p:0.08 and ORGA vs GG:0.65; 95%CI: 0.27-1.55; p:0.33);. Asidethe transition of G to A at codon 54 of FABP2 were not increased psoriasis risk in statistically significant manner insert ignore into journalissuearticles values(OR: 0.68;95% CI: 0.47-0.99; p: 0.046);. The overall odds ratio was found 0.697 insert ignore into journalissuearticles values(p=0.055); by Armitage’s Trend Test. We found no correlation between allelic distribution or genotype frequency of FABP2 Ala54Thr polymorphism and psoriasis. In conclusion, our present results suggest that FABP2 genotypes were not statistically significant and unsuitable to use as dependent determinants of the risk. The alanine to threonine substitution of FABP2 gene can’t be used as diagnostic marker for psoriasis and validation of these findings require to replicate within largescale study group to find real association.
Keywords : Ala54Thr, FABP2, Psoriasis, rs1799883

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