Effects of aspirin on oxidative and nitrosative stress in vascular endothelial cell cultures

Authors : Gunnur DEMİRCAN, Emine DİRAMAN, Yeliz YİLMAZ MİROGLU, Sabri DEMİRCAN, Zafer YAZİCİ, Ahmet Yilmaz COBAN, Tuba YİLDİRİM, Ayhan BİLİR

Pages : 129-137

View : 42 | Download : 9

Publication Date : 2017-01-06

Article Type : Research Paper

Abstract :  In this study, it was aimed to investigate that whether any change in activity of catalase insert ignore into journalissuearticles values(CAT);, superoxide dismutase insert ignore into journalissuearticles values(SOD);, glutathione peroxidase insert ignore into journalissuearticles values(GSH-Px);, lipid peroksidase and nitric oxide insert ignore into journalissuearticles values(NO); over time, and whether any difference between the different doses by giving different doses of aspirin to endothelial cells. Endothelial cells insert ignore into journalissuearticles values(HUVEC); in 24 wells microplates used in this study and 25, 50, 100, 250, 500, 750, 1000 and 1500 μM aspirin to 2 of 4 wells at each row were given, the other 2 wells were included as controls. Accordingly, while CAT, SOD, GSH-Px levels and lipid peroxidation were being measured, NO release from cell media was observed. The significant differences were not found between the baseline insert ignore into journalissuearticles values(0 hour); CAT, SOD, GSH-Px and lipid peroxidase levels measured from lisates that obtained from the cells that different drug doses given and controls insert ignore into journalissuearticles values(p>0.05);. Also, CAT, SOD, GSH-Px and lipid peroxidase levels at 24 insert ignore into journalissuearticles values(p>0.05);, 48 and 72 hours did not show any difference among different drug doses and control insert ignore into journalissuearticles values(p>0.05);. In the control group significant differences were found between CAT, SOD and GSH-Px levels measured at 0, 24, 48, 72 hours insert ignore into journalissuearticles values(p<0.05, p<0.05 and p<0.05 respectively); but lipid peroxidase activity and NO levels showed no difference. Increase in antioxidant enzyme activity in the cells that aspirin was not given, caused by raised free radical formation due to increase in number of cells by time was observed. Aspirin prevented the increase in reactive enzyme activity which increases by time. These results suggest that nontoxic doses of aspirin might protect the cells. 
Keywords : Aspirin, Cell celture, Endothelium, Nitrosative Stress, Oxidative Stress

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