Antiproliferative and apoptotic effects of Onobrychis albiflora extract on HCT- 116 cells

Authors : Muhammet Volkan BÜLBÜL, Hanefi ÖZBEK, Ozlem BAHADIR ACIKARA, Serkan OZBILGIN, Burcın ERGENE OZ, Ekın KURTUL, İlknur KESKIN

Pages : 101-109

View : 38 | Download : 11

Publication Date : 2019-11-18

Article Type : Research Paper

Abstract :In this study, the cytotoxic effects of the extracts of methanol: water insert ignore into journalissuearticles values(80:20); prepared from the above-ground parts of the varieties of Onobrychis albiflora Hub.-Mor., Onobrychis argyrea Boiss. subsp. argyrea, Onobrychis galegifolia Boiss. and Onobrychis tournefortii insert ignore into journalissuearticles values(Willd.); Desv. species to HCT-116 cells were investigated. With cytotoxicity analysis that with these species inhibitor concentrations insert ignore into journalissuearticles values(IC50); which resulted in a 50% reduction in the proliferation of HCT-116 cells were identified. In continuation of the study; the antiproliferative and apoptotic effects of Onobrychis albiflora extract on HCT-116 cells were evaluated by Caspase 3, Annexin V / PI Apoptosis / Necrosis analysis, Apopxin Green and 7-AAD Apoptosis / Necrosis analysis. The antiproliferative, apop- totic and necrotic effects of Onobrychis albiflora extract on HCT-116 cells also were determined. 
Keywords : HCT 116, FACS, HEK 293, NECROSİS, APOPTOSİS, COLON CANCER

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