Evaluation of protective effects of mirtazapine and mesna on cisplatin-induced ovarian damage in rats

Authors : Ali Doğukan ANĞIN, Muhammet Ali ORUÇ, Önder SAKİN, Emine Eda AKALIN, Muzaffer ÇIKMAN, Kayhan BAŞAK, Yasemin ALAN, Asuman ORÇUN, Murat ALAN

Pages : 72-81

View : 50 | Download : 11

Publication Date : 2021-04-03

Article Type : Research Paper

Abstract :Evaluation of protective effects of mirtazapine and mesna on cisplatin-induced ovarian damage in rats Objective: To evaluate whether mirtazapine and mesna have protective effects on cisplatin-induced ovarian injury. Methods: A total of 32 female Wistar Albino rats were divided into 4 groups insert ignore into journalissuearticles values(8 rats per group); and included in the study. No medication was administered to the first group; only intervention was that their ovaries were removed and anti-mullerian hormone insert ignore into journalissuearticles values(AMH); values were measured. The second group received intramuscular cisplatin at a single dose of 7.5 mg/kg. The third group received a single dose of 200 mg/kg mesna intraperitoneally, and 30 minutes later, a single dose of 7.5 mg/kg intramuscular cisplatin was administered. The fourth group received oral 30 mg/kg mirtazapine, and 60 minutes later, a single dose of 7.5 mg/kg intramuscular cisplatin was administered. Oral 30 mg/kg mirtazapine was continued for ten days. Ovaries and AMH values of all groups were evaluated at the end of tenth day. Results: In the cisplatin group when compared to normal ovarian tissue total histopathological damage score increased insert ignore into journalissuearticles values(p=0.037);, preantral follicle count decreased insert ignore into journalissuearticles values(p=0.003); and AMH levels decreased insert ignore into journalissuearticles values(p<0.001);. In the cisplatin+mesna group total ovarian damage score was also increased insert ignore into journalissuearticles values(p=0.005);, preantral and antral follicles decreased insert ignore into journalissuearticles values(p<0.001 and p=0.001, respectively);, and AMH levels decreased insert ignore into journalissuearticles values(p<0.001);. In the cisplatin+mirtazapine group, total ovarian damage score insert ignore into journalissuearticles values(p <0.001);, preantral follicle count insert ignore into journalissuearticles values(p = 0.002); and AMH values were decreased insert ignore into journalissuearticles values(p <0.001);. Conclusion: It was concluded that mesna and mirtazapine were not effective in preventing ovarian damage due to cisplatin.
Keywords : Cisplatin, mirtazapine, mesna, anti mullerian hormone, ovary, rats

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