The Investigation of the Changes in the Surface Glycoconjugates Using Two Different Spheroid Models of Breast Cancer Cells and Availability Assessment of These Spheroid Models for Rapid Diagnosis

Authors : Yosun MATER, Günnur DEMİRCAN

Pages : 266-271

View : 47 | Download : 9

Publication Date : 2021-05-01

Article Type : Research Paper

Abstract :The importance of early cancer diagnosis has led to development of many different diagnostic methods. In this context, the studies investigating the presence and amount of sugar residues to use as indicators in the identification of cancer cell type have become prominent. In the present study, sialic acids found on the membrane surfaces of ER insert ignore into journalissuearticles values(+); MCF-7 and ER insert ignore into journalissuearticles values(-); MDA-MB-231 breast cancer cell lines were labeled using three dimensional insert ignore into journalissuearticles values(3D); cell culture insert ignore into journalissuearticles values(Spheroid); model as the closest method to the patient sample, thus its natural environment, among the cell culture methods. These sugar units that play a role in regulation of important immune characteristics such as recognition, binding and metastasis were made visualizable by applying fluorescent-labeled lectins such as FITC-insert ignore into journalissuearticles values(Wheat Germ Agglutinin); specifically binding to sialic acid units insert ignore into journalissuearticles values(GlcNAc, Neu5Ac); including particularly ß-GlcNAc and FITC-insert ignore into journalissuearticles values(Maackia Amurensis-Lectin-1); specifically binding to Galß4GlcNAc type sialic acids. These glycan units were specifically labeled with FITC-insert ignore into journalissuearticles values(Maackia Amurensis-Lectin-1); and FITC-insert ignore into journalissuearticles values(Wheat Germ Agglutinin); and radiation intensities were analyzed relatively. The two different breast cancer cell cultures were compared with respect to change in the amounts of sialic acid residues containing α-2,3- and α-2,6 bonds using fluorescent-labeled lectins. In the present study, we have performed a precise, accurate and rapid determination of the sugar content in the different breast cancer cell surface lines by means of fluorescent-labeled lectins and carried out a relative comparison between the micrographs.
Keywords : Breast cancer, Spheroid, Sialic acid

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