The Importance of Aminoguanidine and Methylprednisolone Administration in Lung Contusion after Chest Trauma

Authors : Fatih ÇALIŞKAN, Hızır Ufuk AKDEMİR, Celal KATI, Latif DURAN, Tolga GÜVENÇ

Pages : 504-510

View : 38 | Download : 13

Publication Date : 2021-10-09

Article Type : Research Paper

Abstract :ABSTRACT Background: This study aims to evaluate the effect of the antioxidant and antiinflammatory properties of aminoguanidine and metylprednisolone insert ignore into journalissuearticles values(MP); on lung tissue in a pulmonary contusion model of rats and evaluate whether their combined use improves treatment efficacy. Methods: This study included 35 female Sprague Dawley rats weighing 250-300 grams. The rats were divided into five groups as following: Sham; Pulmonary Contusion insert ignore into journalissuearticles values(PC);; PC+MP, PC group treated with i.p methylprednisolone; PC+AG, PC group treated with i.p Aminoguanidine; and PC+AG+MP, PC group treated with Aminoguanidine and methylprednisolone. Each group had seven animals. Blood and lung tissues were studied biochemically and histopathologically. Results: When compared groups according to serum levels of biomarkers, serum YKL-40, nitrate-nitrite, catalase, and TBARS levels were significant different. Serum YKL-40 levels were decreased after treatments in three groups. The serum YKL-40 levels in PC+AG group were lower than the other treatment groups, especially compared to PC + MP insert ignore into journalissuearticles values(p=0,028);. Serum nitrate-nitrite levels were decreased in all treatment groups insert ignore into journalissuearticles values(PC+MP, PC+AG and PC+MP+AG);. The lowest levels were measured in PC+MP+AG; but there was no statistically significant difference compared to PC group insert ignore into journalissuearticles values(p>0.05);. Serum catalase levels were increased in all treatment groups. The higher levels were measured in PC+MP+AG than the other single treatment groups; however PC+MP+AG and PC+MP were statistically significant different compared to PC group insert ignore into journalissuearticles values(p=0,001 and p=0,002 respectively);. Serum TBARS levels were decreased in all treatment groups compared to Sham group insert ignore into journalissuearticles values(p<0.001); and PC group insert ignore into journalissuearticles values(p<0.001);. The lowest levels were measured in PC+MP+AG compared to PC group insert ignore into journalissuearticles values(p<0.001);. Histopatologic and immunohistochemical staining scores were decreased at all the treatment groups, especially PC+MP+AG. Conclusions: We suggest the use of combined treatment of methylprednisolone and aminoguanidine for the treatment of pulmonary contusion.
Keywords : oxidative stress, lung contusion, aminoguanidine, methylprednisolone

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