Investigation of anti neutrophil cytoplasmic antibody presence with indirect immunofluorescence and enzyme-linked immunosorbent assay

Authors : Demet GÜR VURAL, Yeliz TANRIVERDİ ÇAYCI, Kemal BİLGİN, Çağrı ÇOBAN, Asuman BIRINCI

Pages : 313-315

View : 47 | Download : 12

Publication Date : 2022-03-18

Article Type : Research Paper

Abstract :Anti-neutrophil cytoplasmic antibodies insert ignore into journalissuearticles values(ANCA); are a family of autoantibodies that react with proteins expressed mainly in cytoplasmic granules of polymorphonuclear neutrophil granulocytes insert ignore into journalissuearticles values(PMNs);. The ANCA test is used to diagnose small vessel vasculitis and to monitor inflammatory activity. ANCA was initially detected using indirect immunofluorescence insert ignore into journalissuearticles values(IIF);, which allowed differentiation of different patterns, such as p-ANCA insert ignore into journalissuearticles values(perinuclear); and c-ANCA insert ignore into journalissuearticles values(cytoplasmic);. It is now common to detect antibodies by immunochemical assays using purified proteins, such as Enzyme-Linked Immunosorbent Assay insert ignore into journalissuearticles values(ELISA);. In our study, we evaluated ANCA test results studied with IIF and ELISA methods and recorded patient diagnoses in the system. Serum samples of 4524 patients who were thought to have autoimmunity in their etiology were evaluated for ANCA presence. In accordance with the recommendation of the manufacturer insert ignore into journalissuearticles values(Euroimmun AG, Lübeck, Germany);, serum IFA technique was evaluated for the presence of p-ANCA, c-ANCA. ELISA test insert ignore into journalissuearticles values(Alegria, Orgentec); was used to detect antibodies against MPO and PR3. The number of ANCA IIF positive patients was 525insert ignore into journalissuearticles values(11.6%);. When we look at the distribution of ANCAs, 275insert ignore into journalissuearticles values(52.5%); formalin sensitive pANCA, 95 insert ignore into journalissuearticles values(18%); formalin resistant pANCA, 60 formalin sensitive cANCAinsert ignore into journalissuearticles values(11.5%);, 95insert ignore into journalissuearticles values(18%); formalin resistant cANCA. 18 insert ignore into journalissuearticles values(3.4%); of ANCA IIFA positives had PR3 antigen and 22 insert ignore into journalissuearticles values(4.1%); had significant antibody elevation against MPO antigen., ANCA IIF positive samples, according to the diagnosis of information registered in the operating system, consist of 424 insert ignore into journalissuearticles values(80.8%); autoimmune and inflammatory diseases according to disease groups, 36 insert ignore into journalissuearticles values(6.9%); malignancies, 18 insert ignore into journalissuearticles values(3.4%); infectious diseases, 47 insert ignore into journalissuearticles values(8.9%); are other diseases which are not included in these groups. ANCA is a determinant for many diseases, especially vasculitis. ANCA, which we found in a large number of different disease groups, was found to be an indicator that should be used in the diagnosis and follow-up of many autoimmune and inflammatory diseases.
Keywords : Antineutrophilcytoplasmic antibodies, immunofluorescence, proteinase 3, myeloperoxidase

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