The Effects of ATP sensitive potassium channel (KATP) opener and blockers on Bcl-2, Bax, and Cyt-c gene expression levels in epileptic rats

Authors : Ümit KILIÇ, Hayriye SOYTÜRK

Pages : 857-862

View : 35 | Download : 8

Publication Date : 2022-08-30

Article Type : Research Paper

Abstract :In this study, it was aimed to investigate the gene expression levels of Bcl-2, Bax, and cytochrome c insert ignore into journalissuearticles values(Cyt-c);, in the cortex region of pinacidil as a KATP channel opener and glibenclamide as a blocker on penicillin model epilepsy. Male Wistar-Albino rats were used. A total of 4 main groups were formed: Control, Epilepsy, Epilepsy-opener, and Epilepsy-blocker groups, then three-time points were formed subgroups insert ignore into journalissuearticles values(1st day, 4th, and 8th);. 48 rats were used in total. The epileptic focus was created by intracortical administration of penicillin at a dose of 500 IU/2 μl. Cortex is removed from all animals and cyt c, Bcl-2, and Bax gene expression levels were determined by qPCR. The SPSS 21 program was used for statistics. Bcl-2 and Bax gene expression levels were increased in the cortex regions of rats with epilepsy insert ignore into journalissuearticles values(p<0.05);. Bcl-2, Bax gene expression levels, which increased due to epilepsy with the effect of KATP channels opened with pinacidil, returned to normal levels in the epilepsy opener group insert ignore into journalissuearticles values(p<0.05);. Bcl-2 gene expression level, which was increased as a result of epilepsy due to the effect of KATP channels closed with glibenclamide, was higher than in the control and epilepsy-opener groups insert ignore into journalissuearticles values(p<0.05);. Bcl-2 and Bax gene expression levels are increased in the cortex region due to epilepsy indicates that the apoptotic pathway could be activated. This study also It has been shown that the apoptotic pathway activated by epilepsy can be inactivated by pinacidil.
Keywords : Epilepsy, Bcl 2, Bax, Cytochrome c, KATP channels

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