Comparative Clinical and Serum Biochemical Evaluation of Two Intramuscular Anaesthetic Combinations (Diazepam/Ketamine and Diazepam/Fentanyl/Ketamine) in Rabbits

Authors : Mst Antora AKTER, Nelema YESMİN, Md Bayazid Amin TALUKDER, Md Mahmudul ALAM

Pages : 106-114

Doi:10.53518/mjavl.1113511

View : 25 | Download : 18

Publication Date : 2022-12-26

Article Type : Research Paper

Abstract :The research has been conducted to find the clinical efficacy and safety of preanaesthetics and induction agents based on clinical and serum biochemical indicators in rabbits. Eight healthy rabbits insert ignore into journalissuearticles values(3.0-3.5kg); of either sex were arbitrarily allocated into two groups: Group DK insert ignore into journalissuearticles values(diazepam-ketamine); and Group DFK insert ignore into journalissuearticles values(diazepam-fentanyl-ketamine);. Rabbits were received intramuscular diazepam insert ignore into journalissuearticles values(5mg/kg);, fentanyl insert ignore into journalissuearticles values(0.02 mg/kg); and ketamine insert ignore into journalissuearticles values(35 mg/kg);. Data on clinical parameters insert ignore into journalissuearticles values(rectal temperature, heart rate, and respiratory rate); and reflexes insert ignore into journalissuearticles values(righting reflex, palpebral reflex, and pedal reflex); were evaluated before and at 10, 20, 30, 40, 50, 60, 70, 80, 90, and 100 minutes after injection of anaesthetics. Blood samples were aspirated before anaesthesia and 30 minutes following induction. Serum Albumin, Globulin, Cholesterol, Triglyceride, Total Protein, High-Density Lipoprotein insert ignore into journalissuearticles values(HDL);, Low-Density Lipoprotein insert ignore into journalissuearticles values(LDL);, Creatinine, Calcium, Phosphorus, Sodium, Potassium, and Chlorine were evaluated through a semiautomatic biochemistry analyzer. In this study, no significant changes in rectal temperature were observed in the animals of group DK during anaesthesia. During anaesthetic cascade, heart rates and respiration rates in both the groups were reduced significantly insert ignore into journalissuearticles values(P< 0.05);. In DFK-injected rabbits, the return of righting and palpebral reflexes were delayed. The DFK groups had considerably insert ignore into journalissuearticles values(P< 0.05); longer surgical anaesthesia than the DK groups. In terms of serum biochemistry, DK-treated rabbits had significantly lower total protein, globulin, creatinine, HDL, sodium, and potassium concentrations insert ignore into journalissuearticles values(P< 0.05);. Total protein, cholesterol, HDL, and potassium levels decreased significantly insert ignore into journalissuearticles values(P<0.05); after DFK treatment. The DFK combination offered appropriate anaesthesia for rabbits, as evidenced by a prolonged anaesthetic time, acceptable cardiopulmonary and other clinical indices, and moderate changes in serum biochemical profiles.
Keywords : Anaesthesia, ketamine, diazepam, fentanyl, biochemistry, rabbit